Fachbereich Veterinärmedizin



    Time-dependent mRNA expression of cyclooxygenase-2, haptaglobin, interleukin 1-beta and interleukin 8 in the endometrium of postpartum cows (2007)

    Gabler, C.
    Fischer, C.
    Drillich, M.
    Heuwieser, W.
    Einspanier, R.
    40th Annual Conference of Physiology and Pathology of Reproduction and 32nd Mutual Conference on Veterinary and Human Reproductive Medicine
    Berlin, 22. – 23.02.2007
    Reproduction in Domestic Animals, 42, Suppl. 1 (2007), 8
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    Abstract / Zusammenfassung

    Inflammatory processes and infections of the uterine wall
    must be accepted as a event in dairy cows after calving.
    This might result in clinical or subclinical endometritis
    which is supposed to impair reproductive performance in
    the current lactation. Several cytokines and acute phase
    proteins were discussed as local and systemic mediators of
    these inflammatory processes. The aim of the present study
    was to investigate the endometrial mRNA expression of
    cyclooxygenase 2 (COX-2), haptoglobin, interleukin 1-beta
    (IL-1b) and IL-8 in the postpartum period. Endometrial
    cells were obtained on day 10, 17, 24, 31, 38 and 45
    postpartum (p.p.) using the cytobrush®-technique.
    Cytological smears were prepared from cytobrush-samples
    to determine the number of polymorphonuclear neutrophils
    (PMN). Total RNA was extracted from endometrial
    samples and real-time RT-PCR was performed. A timedependent
    mRNA expression of the investigated factors
    was found in the course of the postpartum period. In detail,
    a significant higher expression of these factors was
    observed on day 17 p.p. compared to day 31 p.p. Only
    haptoglobin mRNA was detected in the samples on day 45
    p.p. on the same level as on day 31 pp. This is in contrast to
    the other factors, which were barely detectable on day 45
    p.p. Furthermore, the percentage of PMN decreased from
    day 17 p.p. (> 5%) to low percentages (< 5%) on day 31
    p.p. and decreased thereafter. These results showed that
    inflammatory cytokines and acute phase proteins were
    expressed in the bovine endometrium in a time-related
    manner during the postpartum period. This supports the
    hypothesis that cows with a subclinical or clinical
    endometritis may posses a dysregulated cytokine profile in
    endometrial tissue, followed by failure to implant the