Publikationsdatenbank

Time-dependent mRNA expression of cyclooxygenase-2, haptaglobin, interleukin 1-beta and interleukin 8 in the endometrium of postpartum cows (2007)

Art

Vortrag

Autoren

Gabler, C.

Fischer, C.

Drillich, M.

Heuwieser, W.

Einspanier, R.

Kongress

40th Annual Conference of Physiology and Pathology of Reproduction and 32nd Mutual Conference on Veterinary and Human Reproductive Medicine

Berlin, 22. – 23.02.2007

Quelle

Reproduction in Domestic Animals, 42, Suppl. 1 (2007), 8

Kontakt

Tierklinik für Fortpflanzung

Königsweg 65
Haus 27
14163 Berlin
+49 30 838 62618
fortpflanzungsklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Inflammatory processes and infections of the uterine wall
must be accepted as a event in dairy cows after calving.
This might result in clinical or subclinical endometritis
which is supposed to impair reproductive performance in
the current lactation. Several cytokines and acute phase
proteins were discussed as local and systemic mediators of
these inflammatory processes. The aim of the present study
was to investigate the endometrial mRNA expression of
cyclooxygenase 2 (COX-2), haptoglobin, interleukin 1-beta
(IL-1b) and IL-8 in the postpartum period. Endometrial
cells were obtained on day 10, 17, 24, 31, 38 and 45
postpartum (p.p.) using the cytobrush®-technique.
Cytological smears were prepared from cytobrush-samples
to determine the number of polymorphonuclear neutrophils
(PMN). Total RNA was extracted from endometrial
samples and real-time RT-PCR was performed. A timedependent
mRNA expression of the investigated factors
was found in the course of the postpartum period. In detail,
a significant higher expression of these factors was
observed on day 17 p.p. compared to day 31 p.p. Only
haptoglobin mRNA was detected in the samples on day 45
p.p. on the same level as on day 31 pp. This is in contrast to
the other factors, which were barely detectable on day 45
p.p. Furthermore, the percentage of PMN decreased from
day 17 p.p. (> 5%) to low percentages (< 5%) on day 31
p.p. and decreased thereafter. These results showed that
inflammatory cytokines and acute phase proteins were
expressed in the bovine endometrium in a time-related
manner during the postpartum period. This supports the
hypothesis that cows with a subclinical or clinical
endometritis may posses a dysregulated cytokine profile in
endometrial tissue, followed by failure to implant the
embryo.