Publikationsdatenbank

Determination of NADH by laser-induced fluorescence spectroscopy: applications in preclinical neuroscience (2007)

Art

Poster

Autoren

Rex, A.

Hamann, M.

Richter, A.

Fink, F.

Fink, H.

Kongress

48th Spring Meeting Deutsche Gesellschaft für Experimentelle und Klinische Pharmakologie und Toxikologie

Mainz, 13. – 15.03.2007

Quelle

Naunyn-Schmiedeberg's archives of pharmacology

Bandzählung: 375

Heftzählung: Suppl 1

Seiten: 57

ISSN: 0028-1298

Sprache

Englisch

Kontakt

Institut für Pharmakologie und Toxikologie

Koserstr. 20
14195 Berlin
+49 30 838 53221
pharmakologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

There is an increasing need for continuously monitoring changes in brain metabolism
and neuronal activity, respectively. The majority of the energy, which is produced in
neurons, is necessary for the maintenance of the physiological neuronal activity. The
extent of the energy production is thus closely coupled to the neural activity.
Measurement of NAD(P)H fluorescence by laser-induced fluorescence spectroscopy
and with small glass fibre probes allows the determination of the mitochondrial activity,
and therefore neuronal activity indirectly, in the brain with high temporal and spatial
resolution (Rex & Fink, 2006, Las.Phys.Lett.,3:452-9). In vitro, dependence between the
NADH concentration and NADH fluorescence was proven. Ex vivo investigations
showed that under the selected spectroscopic conditions predominantly NADH
fluorescence contributes to the fluorescence of the tissue and that the fluorescence
intensity differs between brain regions. We could show in anaesthetized rats that the
fluorescence intensity of NADH in the cortex is inversely proportional to the metabolic
activity and that changes in the NADH fluorescence due to haemodynamical effects
altering the optical properties of the tissue can be excluded. In further in vivo
experiments administration of a serotonin 1A receptor agonist with known anxiolytic
activity and inhibitory effects on neuronal activity in the hippocampus causes a
reversible increase in the intensity of hippocampal NADH fluorescence in
pharmacologically relevant doses. In awake and freely moving mutant dtsz hamsters, a
model of paroxysmal dystonia, we could measure reversible changes of the NADH
fluorescence during a dystonic episode. The magnitude of the change corresponds to
the severity of the dystonic episode. The laser-induced fluorescence spectroscopy of the
intrinsic and mainly mitochondrial bound NADH is a versatile applicable and reliable
method which allows the spatial and temporal characterization of the metabolic state
and thus the neuronal activity in the brain in vitro and in vivo