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Background:
Reliable mitotic figure identification is essential for mitotic count assessment in histological tumour sections but shows substantial observer variability. Artificial intelligence (AI) has been shown to improve reproducibility, but AI-generated suggestions may bias pathologists' interpretation. This study evaluated whether veterinary pathologists shift their judgements toward displayed AI-attributed confidence scores when assessing mitotic figures in canine cutaneous mast cell tumours.
Materials & Methods:
Fourteen pathologists scored 500 H&E image patches, each containing a mitotic figure candidate, over three rounds on a 0–100 scale. Scores >50 indicated a mitotic figure and scores <50 indicated no mitotic figure. Two unaided rounds assessed intra-rater variability. In the final round, AI-attributed confidence scores were displayed with the images. These scores were not calculated by a real AI model but were based on each participant's previous ratings and systematically shifted by +/-15, +/-25, or +/-35 points.
Results:
The two unaided rounds showed moderate agreement (intraclass correlation coefficient [ICC] = 0.648, 95% confidence interval [CI] 0.634–0.661), and median absolute intraobserver variability was 10 points. In the AI-supported round, participants shifted their ratings significantly toward the displayed AI-attributed values, with larger discrepancies causing larger shifts in the same direction (beta = 0.118, 95% CI 0.101–0.136, p < 0.001). However, this did not increase binary classification switches beyond unaided variability (22.8% vs. 20.3%).
Conclusion:
AI-attributed confidence scores caused a statistically significant but small anchoring effect on veterinary pathologists' assessments. However, the results indicate that the AIassisted approach did not negatively impact the pathologists' ability to interpret mitotic figure morphologies.