Publication Database

Spatio-temporal diversity and genetic architecture of pyrantel resistance in Cylicocyclus nassatus, the most abundant horse parasite (2025)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Sallé, Guillaume

Courtot, Élise

Cabau, Cédric

Parrinello, Hugues

Serreau, Delphine

Reigner, Fabrice

Gesbert, Amandine

Jacquinot, Lauriane

Lenhof, Océane

Aimé, Annabelle

Picandet, Valérie

Kuzmina, Tetiana

Holovachov, Oleksandr

Bellaw, Jennifer

Nielsen, Martin K.

Samson-Himmelstjerna, Georg <<von>> (WE 13)

Valière, Sophie

Gislard, Marie

Lluch, Jérôme

Kuchly, Claire

Klopp, Christophe

Quelle

Peer community journal

Bandzählung: 5

Seiten: Artikel e69 (30 Seiten)

ISSN: 2804-3871

Sprache

Englisch

Verweise

URL (Volltext): https://peercommunityjournal.org/articles/10.24072/pcjournal.571/

DOI: 10.24072/pcjournal.571

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Cyathostomins are a complex of 50 intestinal parasite species infecting horses and wild equids. The massive administration of modern anthelmintic drugs has increased their relative abundance in horse helminth communities and selected drug-resistant isolates worldwide. Cylicocyclus nassatus is the most prevalent and the most abundant species. The tedious identification and isolation of these worms have hampered studies of their biology that remain largely uncharacterised. Here we have leveraged ultra-low input sequencing protocols to build a reference genome for the most prevalent horse strongyle species. Using this resource, we have established the first estimates of its genetic diversity and population structure on a gradient ranging from Ukraine (close to modern horse domestication area) to North America, while capturing a 19th-century snapshot of C. nassatus diversity in Egypt. Our results support a diverse and lowly structured global population. Modern populations displayed lower nucleotide diversity relative to the old North African isolate. We identified the first genetic candidates upon which pyrantel (an anthelmintic drug used in companion animals) selection likely applied in field populations, highlighting previously suspected genes coding for nicotinic acetylcholine receptor subunits, and identifying new candidates showing differential expression in independently evolved Caenorhabditis elegans lines. These results offer a first resource to widen current knowledge on cyathostomin biology, unravel novel aspects of pyrantel resistance mechanisms and provide candidate genes to track pyrantel resistance in the field.