Publikationsdatenbank

Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs (2025)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Proskurnicka, Angelika

Iskra, Mateusz

Wronka, Sylwia

Bakuła, Zofia

Danesi, Patrizia

Rodrigues de Farias, Marconi

Ramos Portilho, Fábio Vinícius

Garcia Ribeiro, Márcio

Rösler, Uwe (WE 10)

Kano, Rui

Malik, Richard

Jagielski, Tomasz

Quelle

Journal of veterinary internal medicine

Bandzählung: 39

Heftzählung: 1

Seiten: Artikel e17173 (7 Seiten)

ISSN: 0891-6640

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/10.1111/jvim.17173

DOI: 10.1111/jvim.17173

Pubmed: 39564964

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background

Protothecosis in dogs is a rare, yet emerging disease, distinguished by its often-aggressive clinical course and high fatality rate. Our study was conducted to enhance treatment protocols for affected dogs by better understanding the genetic diversity and drug resistance patterns of Prototheca species.
Objectives

To identify species and drug susceptibility profiles of an international collection of 28 Prototheca strains isolated from cases of protothecosis in dogs.
Animals

None.
Methods

Retrospective study. Species-level identification was made for isolates from 28 dogs in 6 countries by molecular typing with the partial cytb gene as a marker. For the determination of minimum inhibitory concentrations (MICs) and minimum algicidal concentrations (MACs), the Clinical Laboratory Standards Institute (CLSI) protocol (M27-A3) was used.
Results

Prototheca bovis was the most prevalent species, accounting for 75% (21/28) of the cases, followed by P. wickerhamii (18%; 5/28) and P. ciferrii (7%; 2/28). Of the 6 drugs tested, efinaconazole (EFZ) was the most potent in vitro, with its median MIC and MAC values equal to 0.125 mg/L. The lowest activity was found for fluconazole (FLU), with MIC and MAC medians of 48 mg/L and 64 mg/L, respectively.
Conclusions and Clinical Importance

Our study identifies P. bovis as the species that most frequently causes protothecosis in dogs, which suggests the possibility of cross-species infection from other animals, especially cows. Additionally, it indicates that EFZ could be used in the treatment of infection in the colon.