Publication Database

Development of GFP-expressing infectious clones for PRRSV using TAR cloning for antiviral drug screening (2025)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Zhang, Minze (WE 5)

Qian, Bang

Kunec, Dusan (WE 5)

Veit, Michael (WE 5)

Quelle

npj viruses

Bandzählung: 3

Heftzählung: 1

Seiten: Artikel 66 (17 Seiten)

ISSN: 2948-1767

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s44298-025-00148-3

DOI: 10.1038/s44298-025-00148-3

Pubmed: 40913022

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Porcine reproductive and respiratory syndrome virus (PRRSV), an Arteriviridae family enveloped RNA virus, is a major swine pathogen. Using yeast transformation-associated recombination (TAR) cloning, we efficiently generated infectious PRRSV and GFP-expressing clones, identifying transcription-regulating sequences as essential for stable foreign gene expression. Screening SARS-CoV-2 antivirals showed potent inhibition by the multitarget drug ribavirin, the polymerase inhibitors remdesivir and its metabolite GS-441524. Molnupiravir, targeting the polymerase by a different mechanism, showed reduced efficacy against PRRSV, while the protease inhibitor GC376 was ineffective. The AlphaFold-predicted structure of the PRRSV polymerase revealed conserved catalytic architecture with the SARS-CoV-2 polymerases, explaining cross-family inhibitor activity. In contrast, structural divergence in proteases correlated with GC376’s inefficacy. These findings underscore the utility of the TAR cloning for arterivirus engineering, with potential applications in vector vaccine development.