Publikationsdatenbank

In search of a conditioned place preference test for mice to assess the severity of experimental procedures (2025)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Jaap, A. (WE 11)

Kahnau, P.

Lewejohann, L. (WE 11)

Quelle

Behaviour : an international journal of behavioural biology

Bandzählung: AOP

Seiten: 1 – 68

ISSN: 1568-539x

Sprache

Englisch

Verweise

URL (Volltext): https://brill.com/view/journals/beh/aop/article-10.1163-1568539X-bja10332/article-10.1163-1568539X-bja10332.xml

DOI: 10.1163/1568539X-bja10332

Kontakt

Institut für Tierschutz, Tierverhalten und Versuchstierkunde

Königsweg 67
14163 Berlin
+49 30 838 61146
tierschutz@vetmed.fu-berlin.de

Abstract / Zusammenfassung

To compare the severity of experimental procedures and behavioural tests from an animal’s perspective, novel methods are required. Theoretically, one feasible approach could be the use of a conditioned place preference test (CPP). This test employs the preference for a certain area in a test apparatus being associated with an experimental treatment. Traditionally, the CPP is used to investigate, for example, the effects of drugs. Instead, we aimed to develop a protocol that would enable us to compare the effects of different experimental procedures conducted with mice. Nine experiments with C57BL/6J mice were performed, varying the setup, the procedure duration, the stimuli as well as the presentation order. None of the tested protocols resulted in a distinct preference. Moreover, even simple protocols using food reward as a treatment failed to result in a conditioned place preference. In summary, none of the protocols was sufficient to form a reliable association between conditioned and unconditioned stimulus. We have scrutinized the experimental setup in detail, and we cannot present a solution yet. However, hopefully, our findings will help to create a working CPP to compare the severity of different experimental procedures for mice.