Publikationsdatenbank

A Comparison of Different Isolation Methods forUrine-Derived Epithelial Cells in Dogs and Horses (2025)

Art

Poster

Autoren

Hollender, A.-C. (WE 17)

Droessler, Linda (WE 2)

Trappe, Susanne (WE 2)

Rausch, Sebastian (WE 6)

Ockler, T. (WE 2)

Stage, Hannah J. (WE 17)

Gehlen, H. (WE 17)

Amasheh, Salah (WE 2)

Kongress

IUPS 2025

11. – 14.09.2025

Quelle

Sprache

Englisch

Kontakt

Pferdeklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62299 / 62300
pferdeklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

A Comparison of Different Isolation Methods for Urine-derived
Epithelial Cells in Dogs and Horses
A. C. Hollender1, 2, L. Droessler1, S. Trappe1, S. Rausch3, T. Ockler1, 2, H. Stage2, H. Gehlen2, S. Amasheh1
1 Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universität Berlin, Berlin, Berlin, Germany
2 Equine Clinic, School of Veterinary Medicine, Freie Universität Berlin, Berlin, Berlin, Germany
3 Institute of Immunology, School of Veterinary Medicine, Freie Universität Berlin, Berlin, Berlin, Germany
Introduction
The culture of primary kidney epithelial cells usually involves tissue digestion after nephrectomy. Urinederived
epithelial cells (UECs) offer a non-invasive source of urinary tract epithelial cells. To optimize yield
and purity, isolation methods for equine and canine UECs were compared.
Methods
Urine samples from dogs (n = 74) were collected from spontaneous midstream urine. Equine samples (n =
21) were collected during general anesthesia induction through a routine urinary catheter. All urine
samples were examined by dipstick, microbiological culture, and microscopy for crystals and cell density.
Cells were seeded onto either gelatine- or Matrigel-coated (1:25) well plates and cultured at 37 °C and 5%
CO₂ for 14 days. Medium exchange and microscopic examination were performed daily. Cells were
passaged at 80–100% confluence until cryopreservation or characterization.
Results
UECs were successfully cultured from 28.6% of equine urine samples. Gelatine-coating showed a 37.5%
and Matrigel-coating a 25.0% success rate. Canine UECs were cultured in 8.1% of cases, with gelatinecoating
showing a 4.6% and Matrigel-coating a 9.6% success rate. Bacterial or fungal contamination
terminated 24.3% of canine and 19.1% of equine cultures. The addition of 0.1% Y-27632, 0.2% primocin,
and 15% FBS to the culture medium showed the most promising growth.
Conclusions
Isolation of equine UECs using gelatine-coated wells and medium containing 15% FBS and 0.1% Y-27632
shows promising results. Isolation of UECs from dogs remains challenging due to contamination and nongrowth,
requiring further optimization.