Publikationsdatenbank

Short-chain fatty acid concentrations in piglets fed different fibre sources (2025)

Art

Vortrag

Autoren

Saliu, Eva-Maria (WE 4)

Ebersbach, Luisa (WE 4)

Roschewitz, Vanessa (WE 4)

Schulze Holthausen, Johannes (WE 4)

Martínez‑Vallespín, Beatriz (WE 4)

Krüsselmann, Philip (WE 4)

Vahjen, Wilfried (WE 4)

Zentek, Jürgen (WE 4)

Grześkowiak, Lukasz (WE 4)

Kongress

29th Congress of the European Society of Veterinary & Comparative Nutrition ESVCN

Leipzig, 04. – 06.09.2025

Quelle

Proceedings of the 29th Congress of the European Society of Veterinary & Comparative Nutrition ESVCN : 4th – 6th September 2025 Leipzig, Germany — Wilkens MR, Vervuert I, Heilmann R, Dobenecker B, Rackwitz R (eds.) (Hrsg.)

Leipzig: Müller Buchbinderei GmbH, 2025. LBH: Leipziger Blaue Hefte — S. 142

Sprache

Englisch

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: Hindgut fibre fermentation produces short-chain fatty acids (SCFA), which contribute to the energy supply of the host. While different dietary fibre sources affect SCFA concentrations in the colon digesta differently [1], little is known about the relationship between colon content SCFA concentration, epithelial SCFA uptake and SCFA blood concentrations. We hypothesised that weaning age, dietary fibre source as well as its particle size affect SCFA formation and translocation in the hindgut of piglets.
Animals, material and methods: 94 piglets (German landrace) were weaned after 4 (CW) or 6 (LW) weeks and fed creep feed and weaner diets containing coarse or finely ground meadow hay or sugar beet pulp (SBP) as fibre source. The piglets were sacrificed 2 weeks after weaning. Blood from the portal vein as well as colon content and tissue were collected. SCFA concentrations were measured in the blood and colon content. SCFA translocation was measured in ussing chamber experiments with colon tissue. Caco-2 cells were used to obtain cell mortality, transepithelial resistance (TEER) and gene expression (MCT-1, MCT-4) when different concentrations and ratios of SCFA were added. Two-way ANOVA (animal trial) and Kruskal-Wallis (Caco2) were performed (p<0.05). The animal trial was approved by the Regional Office for Health and Social Affairs (LAGeSo StN. 014/22).
Results: The highest acetate concentrations were measured in blood from LW piglets, while the opposite was observed for propionate. No age depending differences were detected regarding butyrate blood concentrations. In LW piglets, acetate concentrations were higher in the colon content when SBP was added to the diet (p=0.020). Fibre source and fibre particle size did not influence SCFA concentrations in the blood. In colon contents of CW piglets, acetate and propionate concentrations were higher when fed diets containing hay (p=0.020 and p=0.005, respectively). Diet depending differences in SCFA absorption were only detectable in CW piglets. Here, serosal appearance of acetate (p= 0.033) and propionate (p=0.049), as well as n-butyrate disappearance from the mucosal side (p=0.049) were higher in animals fed SBP containing diets. Adding butyrate or propionate to Caco-2 cells resulted in an upregulation of MCT-1, while the addition of acetate reduced TEER, but no differences were observed regarding cell mortality.
Conclusion: SCFA production and metabolism in the hindgut of piglets differed when fed different fibre sources and particle sizes. This also depended on the weaning age.