Publication Database

Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival (2025)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Jayaram, Anahita

Galande, Sanjeev

Sharbati, Soroush (WE 3)

Deshpande, Deepak

Pawar, Kamlesh

Quelle

RNA Biology

Seiten: 1

Sprache

Englisch

Verweise

URL (Volltext): https://www.tandfonline.com/doi/full/10.1080/15476286.2025.2525886

DOI: 10.1080/15476286.2025.2525886

Pubmed: 40575931

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Monocytes, a type of leukocytes, are key players in immune responses, transitioning to macrophages at infection sites. Differentiation, which is crucial for macrophage survival and resistance to apoptosis, is a tightly regulated process. Based on our earlier findings implicating the 5′-fragment of tRNAHisGUG (5′-HisGUG) in macrophage biology, we specifically focused on these fragments to investigate its regulation during monocyte-to-macrophage differentiation and its role in macrophage survival. We performed small RNA sequencing during monocyte-to-macrophage differentiation using THP-1 cells and identified 5′-HisGUG as a prominently regulated tDR. Notably, transfection of 5′-HisGUG in macrophages decreased survival under apoptotic stress. We propose that 5′-HisGUG can also act as an obstructive RNA (oRNA), and its reduction is critical for macrophage survival under stress.