Publication Database

Tracing ESBL-/pAmpC-producing Escherichia coli in conventional broiler farms during cleaning and disinfection procedures using whole genome sequencing (2024)

Art

Vortrag

Autoren

Robé, Caroline (WE 10)

Projahn, Michaela (WE 10)

Boll, Katrin (WE 10)

Blasse, Anja (WE 10)

Merle, Roswitha (WE 16)

Roesler, Uwe (WE 10)

Friese, Anika (WE 10)

Kongress

21st International Society for Animal Hygiene Conference

Chiang Mai, Thailand, 18. – 20.09.2024

Quelle

The 21th International Society for Animal Hygiene Conference : The 21st International Society for Animal Hygiene Conference : one health in action – innovations in health, welfare and environment for a sustainable animal production : conference proceedings 18-20 September 2024 — International Society for Animal Hygiene (Hrsg.)

— S. 134

Sprache

Englisch

Verweise

URL (Volltext): https://designrr.s3.amazonaws.com/vphcap.res_at_gmail.com_172238/vphcapresgmailcom_ISAH2024-proceedings_FINAL-09271727420633.pdf

Kontakt

Institut für Veterinär-Epidemiologie und Biometrie

Königsweg 67
14163 Berlin
+49 30 838 56034
epi@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background/Objective: Extended-Spectrum Beta-Lactamase- (ESBL-) and plasmid mediated AmpC Beta-Lactamase- (pAmpC-) producing Enterobacterales are frequently detected in broiler farms. Potential transmission routes and intervention measures in the food chain, in the environment and to humans were investigated against ESBL- and pAmpC- producing bacteria, but few data are available on the effects of cleaning and disinfection (C&D) procedures in broiler stables on ESBL- and pAmpC- producing bacteria.
Methods: We systematically sampled five broiler stables before and after C&D using boot swabs, pooled feces samples and gauze swabs. Samples were processed on MacConkey agar with cefotaxime and phenotypically resistant E. coli isolates were further analyzed for their beta-lactam resistance genes and phylogenetic groups, as well as the relation of isolates from the investigated stables before and after C&D by whole genome se-
quencing.
Results: ESBL- and pAmpC- producing E. coli was detected at sites where C&D was not performed or where insufficient cleaning was performed prior to disinfection. Highly related ESBL-/pAmpC- producing E. coli isolates were detected before and after C&D in four of five broiler stables using cgMLST. Survival as well as transmission of resistant isolates in investigated broiler stables or from broiler stables to the anteroom and surrounding environment and between broiler farms was shown.
Conclusion: C&D can reduce ESBL-/pAmpC- producing E. coli in conventional broiler stables. Complete ESBL- and pAmpC- elimination does not seem to be possible in practice as several factors influence the C&D outcome. Multifactorial approaches, combining various hygiene- and management measures are essential to reduce ESBL-/pAmpC- E. coli in broiler farms.