Publikationsdatenbank

Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schermuly, Isabel I. (WE 2)

Romanet, Stella (WE 2)

Patra, Amlan K.

Mastrototaro, Lucia

Lemme, Andreas

Pieper, Robert

Zentek, Jürgen (WE 4)

Aschenbach, Jörg R. (WE 2)

Quelle

Nutrients

Bandzählung: 16

Heftzählung: 19

Seiten: 3418

ISSN: 2072-6643

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2072-6643/16/19/3418

DOI: 10.3390/nu16193418

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background: Methionine (Met) is a popular nutritional supplement in humans and animals. It is routinely supplemented to pigs as L-Met, DL-Met, or DL-2-hydroxy-4-(methylthio) butanoic acid (DL-HMTBA).

Methods: We investigated the effect of these Met supplements on jejunal amino acid (AA) transport in male castrated Piétrain × Danbred pigs, also including a non-supplemented group. The mucosal-to-serosal flux of ten [¹⁴C]-labeled AAs (L-glutamine, glycine, L-leucine, L-lysine, L-Met, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine) was investigated at two concentrations (50 µM and 5 mM). Inhibition of apical uptake by mucosal L-Met was also measured for these AAs. The intestinal expression of apical AA transporters, angiotensin-converting enzyme II and inflammation-related genes were compared with those of a previous study.

Results: Except for tryptophan and lysine at 5 mM, all AA fluxes were Na⁺-dependent (p ≤ 0.05), and the uptake of most AAs, except glycine and lysine, was inhibited by L-Met (p < 0.001). A correlation network existed between Na⁺-dependent fluxes of most AAs (except tryptophan and partly glycine). We observed the upregulation of B⁰AT1 (SLC6A19) (p < 0.001), the downregulation of ATB^0,+ (SLC6A14) (p < 0.001) and a lower expression of CASP1, IL1β, IL8, TGFβ and TNFα in the present vs. the previous study (p < 0.001).

Conclusions: The correlating AAs likely share the same Na⁺-dependent transporter(s). A varying effect of the Met supplement type on AA transport in the two studies might be related to a different level of supplementation or a different inflammatory status of the small intestine.