Publication Database

Measurement of oro-cecal transit time in LPS-treated pigs fed high and low fiber diets using the lactose-13C-ureide test in breath and saliva samples (2025)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Cavalleri, Mariagrazia

Sciascia, Quentin L.

Görs, Solvig

Vernunft, Andreas

Reyer, Henry

Wimmers, Klaus

Zentek, Jürgen (WE 4)

Kluess, Jeannette

Dänicke, Sven

Metges, Cornelia C.

Quelle

Journal of agricultural and food chemistry : a publication of the American Chemical Society

Bandzählung: 73

Heftzählung: 17

Seiten: 10304 – 10315

ISSN: 0021-8561

Sprache

Englisch

Verweise

URL (Volltext): https://pubs.acs.org/doi/10.1021/acs.jafc.5c00534

DOI: 10.1021/acs.jafc.5c00534

Pubmed: 40234424

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The lactose-13C-ureide (L13CU) test, used in humans to measure oro-cecal transit time (OCTT) in breath CO2, was assessed for its suitability in pigs as a noninvasive alternative to intestinal cannulation. The OCTT was determined with the L13CU test in breath and saliva samples when pigs were fed low or high dietary fiber (DF) (low fiber, 2.8% DF; high fiber 6.5% DF) diets, and 24 h after an i.m. injection with either lipopolysaccharide (LPS) or NaCl. The OCTT measured in breath was longer in LF-LPS than in LF-NaCl and HF-LPS groups (3.4 vs 2.9 h; p < 0.05). Additionally, LPS prolonged the OCTT of pigs, and DF prevented this effect. 90 % of OCTT estimates measured in saliva and breath CO2 did not differ. We conclude that the L13CU breath test is useful for determining OCTT in pigs; saliva is generally suitable as a sample matrix for OCTT, but its use requires further validation.