Publikationsdatenbank

Associations between inflammatory processes in the uterus and oviduct in postpartum dairy cows (2025)

Art

Vortrag

Autoren

Habich, E. M.

Neubrand, L.

Tekin, M.

Guse, C.

Pothmann, H.

Havlicek, V.

Besenfelder, U.

Gabler, C. (WE 3)

Drillich, M. (WE 18)

Wagener, K.

Kongress

58th Annual Conference of Physiology and Pathology of Reproduction and 50th Joint Conference of Veterinary and Human Reproductive Medicine

Leipzig, 26. – 28.02.2025

Quelle

Reproduction in domestic animals = Zuchthygiene

Bandzählung: 60

Heftzählung: S1

Seiten: 33

ISSN: 0936-6768

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/10.1111/rda.70016

DOI: 10.1111/rda.70016

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Excessive inflammation in the reproductive tract may adversely affect early embryo development. Therefore, the objective of the study was to investigate the relationship between inflammatory processes in the uterus and oviduct in postpartum dairy cows. Uterine samples were taken by the cytobrush technique and oviductal samples were collected by transvaginal endoscopy from 31 cows at 28 and 56 days postpartum (dpp). Samples were used for cytology to determine the percentage of polymorphonuclear neutrophils (PMN%). In addition, total RNA was extracted for RT-qPCR of selected pro-inflammatory factors (CXCL1/2, CXCL3, CXCL8, IL1A, IL1B, and PTGS2), receptors (TLR2 and TLR4), and OVGP1. Based on uterine PMN% (≥ 5%) and vaginoscopic findings (pus in the discharge) at 28 dpp, cows were classified as “healthy” (HE; n = 17) or “endometritic” (EN; n = 14). At 28 dpp, EN showed 2.4-fold greater uterine PMN% (p < 0.05) and greater mRNA abundance of CXCL1/2, IL1A, IL1B, PTGS2, and TLR4 (2 to 7-fold; p < 0.05) compared to HE. In the oviduct, EN showed significantly greater PMN% (1.6-fold, p < 0.05) and greater mRNA abundance of CXCL1/2 and CXCL3 than HE (both 1.4-fold; p < 0.05) at 28 dpp. Numerous positive correlations were found between the uterine and oviductal mRNA expression at 28 dpp (p < 0.05). Uterine PMN% at 28 dpp correlated significantly with all pro-inflammatory factors in the oviduct at 28 and 56 dpp (p < 0.05). In contrast, negative correlations were found between uterine PMN% and oviductal TLR4 and OVGP1 mRNA expression at 28 dpp. The results indicate pro-inflammatory processes in the oviduct of endometritic cows, which may impair subsequent reproductive events, such as gamete maturation, fertilisation, and early embryo development.