Publikationsdatenbank

Critical assessment of commercially available microtitre panels for antimicrobial susceptibility testing of veterinary pathogens (2025)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schwarz, Stefan (WE 7)

Bäumer, Wolfgang (WE 14)

Feßler, Andrea T. (WE 7)

Jung, Arne

Kaspar, Heike

Kehrenberg, Corinna

Richter, Angelika

Schwarz, Christine

Verspohl, Jutta

Werckenthin, Christiane

Quelle

The journal of antimicrobial chemotherapy : JAC

Bandzählung: 80

Heftzählung: 2

Seiten: 319 – 321

ISSN: 0305-7453

Sprache

Englisch

Verweise

URL (Volltext): https://academic.oup.com/jac/article/80/2/319/7935060?login=true

DOI: 10.1093/jac/dkae471

Pubmed: 39737883

Kontakt

Institut für Pharmakologie und Toxikologie

Koserstr. 20
14195 Berlin
+49 30 838 53221
pharmakologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Antimicrobial susceptibility testing (AST) in the veterinary sector by broth microdilution is mainly based on commercially available microtitre plates with specific panels. A critical review of commercially available microtitre panels identified AST panels that fulfil the requirements for obtaining reliable AST results by covering the necessary antimicrobial concentrations for both clinical breakpoints as well as quality control (QC) ranges for approved QC strains. However, there are AST panels in which these prerequisites are only in part fulfilled, and some AST panels that do not fulfil the aforementioned criteria at all. Most customers believe that commercially available microtitre panels, which are used in microbiological diagnostic laboratories, reliably allow the identification of susceptible, intermediate or resistant bacteria in addition to sufficient QC ranges to validate the test system. Unfortunately, our findings revealed that this assumption is not always true, and thus it is time to reinforce the necessary requirements that microtitre panels must fulfil to give reliable AST data.