Publikationsdatenbank

Through the labyrinth:
how Salmonella penetrates the gastrointestinal mucus (2024)

Art

Poster

Autoren

Ghazisaeedi, F. (WE 7)

Diestelhorst, K.

Klimek, A.

Braetz, S. (WE 7)

Tedin, K. (WE 7)

Weinhart, M.

Netz, R.

Block, S.

Fulde, M. (WE 7)

Kongress

6th International Conference of the European College of Veterinary Microbiology

University of Nottingham, UK, 05. – 07.09.2024

Quelle

Scientific abstracts : 6th International Conference of the European College of Veterinary Microbiology : 5th – 6th September 2024, University of Nottingham, UK

— S. 43

Sprache

Englisch

Verweise

URL (Volltext): https://cdn-eu.eventscase.com/clearwaterevents.eventscase.com/uploads/users/971808/uploads/83159d715ef64b1ca7f9eed01d78e14dcba7cbd9231f1adb81e36986efa9d1aa5ff13e3bf142f1b140448156ca2784a07c89.66d813278d10c.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Motility and virulence of enteropathogenic bacteria such as Salmonella enterica and Escherichia coli have been shown to connected by complex regulatory networks. Apart from motility, flagellum nanomachine can contribute to bacterial chemotaxis and evasion of host immune responses. For successful host
infection, pathogens need to penetrate mucus layer to be able to disrupt the mucosal barrier and invade the epithelial cells in the gut. Previous studies showed the importance of the flagella driven motility in breaching of the mucosal barrier and gut colonization. However, the exact bacterial navigation strategies and mechanisms of interaction between bacteria and mucus layer/underlying cells are unknown.
We aim to elucidate molecular mechanism of interaction between enteric pathogen Salmonella Typhimurium and the gastrointestinal mucus prior to intestinal epithelium invasion with a focus on the role of bacterial flagellar apparatus. Thus, we generated a group of GFP-expressing Salmonella Typhimurim ATCC 14028 mutants lacking different structural, antigenic and functional parts of flagellum such as ΔfliB, ΔfliC and ΔflijB/fliC. To this end, using optical microscopy we follow the motion of Salmonella Typhimurium at interfaces and within biological hydrogels such as mucus. We will give examples, how motility of Salmonella is affected by the properties of its environment or the Salmonella mutant probed.
Furthermore, by continuously recording z-stacks of samples, in which Salmonella interact with mucus-producing cells, we succeeded to follow dynamics of bacterial penetration through a biologically relevant hydrogel. We will show that bacterial penetration predominantly proceeds via hydrogel voids, which either already present or actively generated by bacteria.