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    Comparative phylogenetic and sequence identity analysis of internal transcribed spacer 2 and cytochrome C oxidase subunit I as DNA barcode markers for the most common equine Strongylidae species (2025)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Diekmann, Irina (WE 13)
    Krücken, Jürgen (WE 13)
    Kuzmina, Tetiana A.
    Bredtmann, Christina M. (WE 13)
    Louro, Mariana (WE 13)
    Kharchenko, Vitaliy A.
    Tzelos, Thomas
    Matthews, Jacqueline B.
    Madeira de Carvalho, Luís M.
    von Samson-Himmelstjerna, Georg (WE 13)
    Quelle
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases ; MEEGID
    Bandzählung: 129
    Seiten: 105729
    ISSN: 1567-1348
    Sprache
    Englisch
    Verweise
    URL (Volltext): https://www.sciencedirect.com/science/article/pii/S1567134825000188
    DOI: 10.1016/j.meegid.2025.105729
    Pubmed: 39955017
    Kontakt
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    14163 Berlin
    +49 30 838 62310
    parasitologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Morphologically, 64 strongylid species have been described in equines. Co-infections are common, with up to 29 species reported in a single horse. Morphological identification of these species is time consuming and requires expert knowledge due to their similar appearance. Therefore, non-invasive identification methods are needed. DNA barcoding offers a rapid and reliable tool for species identification and the discovery of cryptic species for these most common parasitic nematodes of equines. In total, 269 COI and 312 ITS-2 sequences from 27 equine Strongylidae species, including sequences from two uncharacterised species, Coronocyclus sagittatus and Triodontophorus tenuicollis, were generated and combined with COI and ITS-2 sequences data from six Cyathostominae species from previous studies. This study represents a comprehensive DNA barcoding analysis of 22 Cyathostominae and six Strongylinae species using mitochondrial cytochrome c oxidase subunit I (COI) gene and internal transcribed spacer 2 (ITS-2) sequences. Maximum likelihood (ML) phylogenetic trees were constructed and the intra- and interspecific genetic distances for both markers were compared. Analysis revealed complex phylogenetic relationships. Para- and polyphyletic relationships were observed among most genera within Strongylinae and Cyathostominae. This challenges current morphological classifications. Although both markers showed overlapping pairwise identities in intra- and inter-species comparisons, COI had higher discriminatory power than ITS-2. Expanding the COI and ITS-2 reference database, including the first sequences for Coronocyclus sagittatus and Triodontophorus tenuicollis, improve a reliable species identification and advanced studies on Strongylinae and Cyathostominae diversity using barcoding and metabarcoding.