Publikationsdatenbank

The gut microbiome, resistome, and mycobiome in preterm newborn infants and mouse pups:
lack of lasting effects by antimicrobial therapy or probiotic prophylaxis (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Yuu, Elizabeth Y.

Bührer, Christoph

Eckmanns, Tim

Fulde, Marcus (WE 7)

Herz, Michaela

Kurzai, Oliver

Lindstedt, Christin

Panagiotou, Gianni

Piro, Vitor C.

Radonic, Aleksandar

Renard, Bernhard Y.

Reuss, Annicka

Siliceo, Sara Leal

Thielemann, Nadja

Thürmer, Andrea

Vorst, Kira van (WE 7)

Wieler, Lothar H. (WE 7)

Haller, Sebastian

Quelle

Gut pathogens : the official journal of The International Society for Genomic and Evolutionary Microbiology (ISOGEM)

Bandzählung: 16

Heftzählung: 1

Seiten: 27

ISSN: 1757-4749

Sprache

Englisch

Verweise

URL (Volltext): https://gutpathogens.biomedcentral.com/articles/10.1186/s13099-024-00616-w

DOI: 10.1186/s13099-024-00616-w

Pubmed: 38735967

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background: Enhancing our understanding of the underlying influences of medical interventions on the microbiome, resistome and mycobiome of preterm born infants holds significant potential for advancing infection prevention and treatment strategies. We conducted a prospective quasi-intervention study to better understand how antibiotics, and probiotics, and other medical factors influence the gut development of preterm infants. A controlled neonatal mice model was conducted in parallel, designed to closely reflect and predict exposures. Preterm infants and neonatal mice were stratified into four groups: antibiotics only, probiotics only, antibiotics followed by probiotics, and none of these interventions. Stool samples from both preterm infants and neonatal mice were collected at varying time points and analyzed by 16 S rRNA amplicon sequencing, ITS amplicon sequencing and whole genome shotgun sequencing.

Results: The human infant microbiomes showed an unexpectedly high degree of heterogeneity. Little impact from medical exposure (antibiotics/probiotics) was observed on the strain patterns, however, Bifidobacterium bifidum was found more abundant after exposure to probiotics, regardless of prior antibiotic administration. Twenty-seven antibiotic resistant genes were identified in the resistome. High intra-variability was evident within the different treatment groups. Lastly, we found significant effects of antibiotics and probiotics on the mycobiome but not on the microbiome and resistome of preterm infants.

Conclusions: Although our analyses showed transient effects, these results provide positive motivation to continue the research on the effects of medical interventions on the microbiome, resistome and mycobiome of preterm infants.