Publikationsdatenbank

Single-cell-resolved interspecies comparison shows a shared inflammatory axis and a dominant neutrophil-endothelial program in severe COVID-19 (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Peidli, Stefan

Nouailles, Geraldine

Wyler, Emanuel

Adler, Julia M.

Kunder, Sandra

Voß, Anne

Kazmierski, Julia

Pott, Fabian

Pennitz, Peter

Postmus, Dylan

Teixeira Alves, Luiz Gustavo

Goffinet, Christine

Gruber, Achim D. (WE 12)

Blüthgen, Nils

Witzenrath, Martin

Trimpert, Jakob

Landthaler, Markus

Praktiknjo, Samantha D.

Quelle

Cell reports

Bandzählung: 43

Heftzählung: 6

Seiten: 114328

ISSN: 2211-1247

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S2211124724006569?via%3Dihub

DOI: 10.1016/j.celrep.2024.114328

Pubmed: 38861386

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

A key issue for research on COVID-19 pathogenesis is the lack of biopsies from patients and of samples at the onset of infection. To overcome these hurdles, hamsters were shown to be useful models for studying this disease. Here, we further leverage the model to molecularly survey the disease progression from time-resolved single-cell RNA sequencing data collected from healthy and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected Syrian and Roborovski hamster lungs. We compare our data to human COVID-19 studies, including bronchoalveolar lavage, nasal swab, and postmortem lung tissue, and identify a shared axis of inflammation dominated by macrophages, neutrophils, and endothelial cells, which we show to be transient in Syrian and terminal in Roborovski hamsters. Our data suggest that, following SARS-CoV-2 infection, commitment to a type 1- or type 3-biased immunity determines moderate versus severe COVID-19 outcomes, respectively.