Publikationsdatenbank

Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Lange, Andreas

Medugorac, Ivica

Ali, Asghar

Kessler, Barbara

Kurome, Mayuko

Zakhartchenko, Valeri

Hammer, Sabine E.

Hauser, Andreas

Denner, Joachim (WE 5)

Dobenecker, Britta

Wess, Gerhard

Tan, Paul L. J.

Garkavenko, Olga

Reichart, Bruno

Wolf, Eckhard

Kemter, Elisabeth

Quelle

Xenotransplantation : the official journal of the International Xenotransplantation Association, a section of The Transplantation Society

Bandzählung: 31

Heftzählung: 2

Seiten: e12858

ISSN: 1399-3089

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/10.1111/xen.12858

DOI: 10.1111/xen.12858

Pubmed: 38646921

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

One of the prerequisites for successful organ xenotransplantation is a reasonable size match between the porcine organ and the recipient's organ to be replaced. Therefore, the selection of a suitable genetic background of source pigs is important. In this study, we investigated body and organ growth, cardiac function, and genetic diversity of a colony of Auckland Island pigs established at the Center for Innovative Medical Models (CiMM), LMU Munich. Male and female Auckland Island pig kidney cells (selected to be free of porcine endogenous retrovirus C) were imported from New Zealand, and founder animals were established by somatic cell nuclear transfer (SCNT). Morphologically, Auckland Island pigs have smaller body stature compared to many domestic pig breeds, rendering their organ dimensions well-suited for human transplantation. Furthermore, echocardiography assessments of Auckland Island pig hearts indicated normal structure and functioning across various age groups throughout the study. Single nucleotide polymorphism (SNP) analysis revealed higher runs of homozygosity (ROH) in Auckland Island pigs compared to other domestic pig breeds and demonstrated that the entire locus coding the swine leukocyte antigens (SLAs) was homozygous. Based on these findings, Auckland Island pigs represent a promising genetic background for organ xenotransplantation.