Publikationsdatenbank

Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Xie, Ning

Ma, Tengfei

Gao, Yuan

Fu, Jiani

Tan, Chune

Wang, Zheng

Wang, Zhuo

Krüger-Haker, Henrike (WE 7)

Feßler, Andrea T. (WE 7)

Wu, Congming

Shen, Jianzhong

Schwarz, Stefan (WE 7)

Wang, Yang

Quelle

Journal of global antimicrobial resistance

Bandzählung: 39

Seiten: 170 – 174

ISSN: 2213-7165

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S2213716524001723?via%3Dihub

DOI: 10.1016/j.jgar.2024.09.004

Pubmed: 39362467

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background: The emergence and transmission of the multidrug resistance gene cfr have raised public health concerns worldwide.

Objectives: Multidrug-resistant Staphylococcus equorum isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one Staphylococcus equorum isolate harbouring two distinct cfr-carrying plasmids.

Methods: Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. De novo hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools.

Results: Staphylococcus equorum isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two cfr-harbouring plasmids: the RepA N-family plasmid pSN42-51 K and the Inc18-family plasmid pSN42-50 K. These two plasmids exhibited low structural similarities to the so far reported cfr-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene lsa(B). In addition, plasmid pSN42-51 K carried two erm(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene ant(6)-Ia as well as mercury resistance genes while pSN42-50 K was associated with the heavy metal translocating P-type ATPase gene hmtp. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the cfr-carrying plasmids.

Conclusion: This is the first report of S. equorum carrying two distinct cfr-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health.