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Colibacillosis is a systemic or localised infection caused entirely or partly by avian pathogenic E. coli (APEC). Infections with APEC are mostly accompanied by high morbidity, high mortality, a high condemnation rate, drop in egg production and drop in hatchability, causing severe economic losses in poultry. The most important systemic infections are colisepticaemia and coligranulomatosis (Hjärre’s disease). However, enteritis, omphalitis (yolk sac inflammation), salpingitis (inflammation of the oviduct), swollen head syndrome (SHS), coliform cellulitis, orchitis, osteomyelitis/synovitis and panophthalmitis are forms of local infections. E. coli strains that cause diseases beyond the intestinal tract of any species are known as extraintestinal pathogenic E. coli (ExPEC), and they share common characteristics. Most APEC are ExPEC and have similar features to mammalian ExPEC. Additionally, infections with M. gallisepticum, avian metapneumovirus, Pasteurella multocida, Erysipelothrix rhusiopathiae and Histomonas meleagridis are usually complicated by secondary bacterial infections with E. coli. Although most APEC strains are non-zoonotic, E. coli O157:H7 is zoonotic and has been isolated from several avian species, including turkeys. The diagnosis of APEC is based on clinical signs, postmortem lesions and bacteriological examination. Most bacterial isolates are known to be multiple antibiotic-resistant, so an antibiogram is recommended before antibiotic treatment. In addition to proper management, prevention depends on the vaccination, with live attenuated or inactivated vaccines against the most common pathogenic serotypes.