Publication Database

Milk production by in-vitro cultured lactocytes? (2024)

Art

Poster

Autoren

Rickmann, K. (WE 1)

Bahramsoltani, M. (WE 1)

Vidak, J. (WE 14)

Filor, V. (WE 14)

Pelckmann, L. (WE 1)

Al-Masri, S. (WE 1)

Kongress

13. Syposium für Doktorandinnen und Doktoranden - 2024

10.10.2024

Quelle

Sprache

Englisch

Kontakt

Institut für Pharmakologie und Toxikologie

Koserstr. 20
14195 Berlin
+49 30 838 53221
pharmakologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: Due to its high nutritional content and its ability to be further processed in different ways, milk is widely consumed and a food of great importance. There is not only an economic interest in developing and establishing alternative milk production options, but also a social interest with regard to climate protection and animal welfare. The study initially aims to develop an in vitro model based on bovine lactocytes, to explore the potential of in vitro cultured lactocytes as a milk source.

Material and Methods: Bovine lactocytes were isolated from the udder of slaughtered heifers and dairy cows and grown in vitro. Isolated cells were cultured in cell culture flasks and on polyester membranes with a pore size of 0.4 μm. They were incubated in a medium containing RPMI1640 medium, fetal calf serum and penicillin-streptomycin. Immunocytochemistry was used to characterize the luminal lactocytes and differentiate them from myoepithelial cells, fibrocytes and endothelial cells based on their expression of butyrophilin, myosin, collagen I and CD34.

Results: Based on the distribution of the staining and the shape of the stained cells, it could be determined that the myosin antibody stained the myoepithelial cells and the collagen I antibody stained the fibrocytes.

Conclusion: The immunocytochemistry performed so far has shown that myosin and collagen I are suitable markers for differentiating the myoepithelial cells and the fibrocytes. In further trials, the butyrophilin antibody and the CD34 antibody will be tested for their suitability to differentiate luminal lactocytes and endothelial cells.