Publikationsdatenbank

Telomeric repeats in the commercial SB-1 vaccine facilitate viral integration and contribute to vaccine efficacy (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

You, Yu

Kheimar, Ahmed M.

Vychodil, Tereza

Kossak, Lisa

Sabsabi, Mohammad A.

Conradie, Andelé M. (WE 5)

Reddy, Sanjay M.

Bertzbach, Luca D.

Kaufer, Benedikt B. (WE 5)

Quelle

npj vaccines

Bandzählung: 9

Heftzählung: 1

Seiten: 154

ISSN: 2059-0105

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s41541-024-00945-6

DOI: 10.1038/s41541-024-00945-6

Pubmed: 39169010

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Marek's disease virus (MDV) integrates its genome into the telomeres of host chromosomes and causes fatal lymphomas in chickens. This integration is facilitated by telomeric repeat sequences (TMRs) at the ends of the viral genome, and is crucial for MDV-induced lymphomagenesis. The SB-1 vaccine virus is commonly used in commercial bivalent vaccines against MDV and also contains TMRs at its ends. Here, we demonstrate that SB-1 efficiently integrates its genome into the chromosomes of latently infected T cells. Deletion of the TMRs from the SB-1 genome did not affect virus replication, but severely impaired virus integration and genome maintenance in latently infected T cells and in chickens. Strikingly, the reduced integration and maintenance of latent SB-1 significantly impaired vaccine protection. Taken together, our data revealed that the TMRs facilitate SB-1 integration and that integration and/or maintenance of the latent viral genome is critical for vaccine protection.