Publikationsdatenbank

Pathogenicity of Arcobacter cryaerophilus (2024)

Art

Poster

Autoren

Bachus, Antonia (WE 8)

Gölz, Greta (WE 8)

Alter, Thomas (WE 8)

Kongress

International One Health Symposium 2024

Berlin, 17. – 19.10.2024

Quelle

One Health Symposium : 17-19 October 2024 : Book of Abstracts

— S. 62

Sprache

Englisch

Verweise

URL (Volltext): https://my.orgalution.de/s/k5CGCrkNgPGz3LE

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The gram-negative bacterium Arcobacter cryaerophilus is associated with gastrointestinal illnesses in infected humans. Due to its occurrence in not only various environmental habitats but also different kinds of food, it is considered an emerging foodborne pathogen. Nevertheless, the pathogenicity mechanisms responsible for symptoms such as watery diarrhea and abdominal cramps remain largely unknown.
Therefore, we tested twelve Arcobacter cryarophilus strains of different origins for their pathogenic potential in the two human intestinal cell lines HT-29/B6 and T84.
We observed that all twelve tested strains were able to adhere to and invade both cell lines on a similar level. Moreover, in a cytotoxicity assay, we measured the decrease in the metabolic activity of infected cells. We observed two of the twelve strains to decrease the metabolic activity to less than 50% residual metabolic activity in both cell lines.
In addition, to detect a decrease in the permeability of the epithelial barrier, we measured the reduction of the transepithelial resistance in T84 cells. Four of five tested strains reduced the transepithelial resistance of the cells to 50% or less.
The various observed pathogenicity mechanisms of A. cryaerophilus in two human colonic cell lines support the suggestion that this bacterium poses a possible hazard to human health and underline the importance of further investigations of the bacterium.