Publikationsdatenbank

Antimicrobial and biocide susceptibility among Staphylococcus aureus and Staphylococcus pseudintermedius from dogs and cats (2023)

Art

Poster

Autoren

Schug, Angela R. (WE 7)

Scholtzek, Anissa D. (WE 7)

Kohn, Barbara (WE 20)

Weingart, Christiane (WE 20)

Schink, Anne-Kathrin (WE 7)

Bethe, Astrid (WE 7)

Lübke-Becker, Antina (WE 7)

Feßler, Andrea T. (WE 7)

Schwarz, Stefan (WE 7)

Kongress

ARAE 2023

Tours, Frankreich, 03. – 05.07.2023

Quelle

9th Symposium on Antimicrobial Resistance in Animals and the Environment : abstracts book

Tours, Frankreich, 2023 — S. 100

Sprache

Englisch

Verweise

URL (Volltext): https://arae2023.symposium.inrae.fr/content/download/770/8117?version=1

Kontakt

Klein- und Heimtierklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62422
kleintierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: Since Staphylococcus aureus and Staphylococcus pseudintermedius have a zoonotic potential, monitoring of their antimicrobial and biocide resistance is an important issue. The aim of the present study was to investigate the resistance situation of canine and feline S. aureus and S. pseudintermedius isolates from diagnostic submissions.
Materials and Methods: A total of 114 isolates, including 62 S. aureus, 52 S. pseudintermedius were investigated for (i) their susceptibility to 27 antimicrobial agents by broth microdilution according to CLSI and the detection of the respective resistance genes. Moreover, the isolates were tested for their susceptibility to four biocides, namely benzalkonium chloride, chlorhexidine, polyhexanide and octenidine by a recently developed biocide susceptibility testing protocol.
Results: Penicillin resistance mediated by the blaZ gene was the dominant resistance property with 80.65% among S. aureus and 86.54% S. pseudintermedius. About one quarter of the isolates (S. aureus 33.87%; S. pseudintermedius 15.38%) proved to be methicillin-resistant and carried the genes mecA or mecC. Macrolide resistance with 27.2% was second most prevalent and all isolates harbored the resistance genes erm(A), erm(B), erm(C), erm(T) or msr(A), alone or in combinations. Among all isolates tested fluoroquinolone resistance was detected in 21.1% and tetracycline resistance mediated by tet(K) and/or tet(M) occurred in 19.3% of the isolates. Resistance to last resort antimicrobial agents for human medicine was only detected in single isolates, if at all. The biocide susceptibility testing showed unimodal minimal inhibitory concentration (MIC) distributions of the four biocides and were very similar for S. aureus and S. pseudintermedius.
Conclusions: While various antimicrobial resistance properties were determined and a considerable number of the isolates displayed a multiresistance phenotype, the biocide susceptibility testing revealed unimodal distributions not pointing towards resistance development. Further monitoring is necessary to early detect changes in the resistance situation.