Publikationsdatenbank

Comparative spatial proteomics between SARS-CoV-2 infected lungs of Roborovski dwarf hamsters and Syrian hamsters (2023)

Art

Vortrag

Autoren

Kunder, S. (WE 12)

Voss, A. (WE 12)

Hempel, B. F. (Tiermedizinisches Zentrum für Resistenzforschung)

Trimpert, J. (WE 5)

Dietert, K. (Tiermedizinisches Zentrum für Resistenzforschung)

Gruber, A. D. (WE 12)

Kongress

European Congress of Veterinary Pathology and Clinical Pathology (ESVP/ECVP/ESVCP/ECVCP)

Lisbon, 30.08. – 02.09.2023

Quelle

Journal of comparative pathology

Bandzählung: 210

Seiten: 51 – 52

ISSN: 0021-9975

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S0021997524000410?via%3Dihub

Kontakt

Tiermedizinisches Zentrum für Resistenzforschung

Robert-von-Ostertag-Str. 8
14163 Berlin

Abstract / Zusammenfassung

Introduction: Of various COVID-19 models, two hamster species are of particular interest. Specifically, the Roborovski dwarf hamster mimics a severe, rapidly lethal pneumonia as seen in highly susceptible patients, whereas the Syrian hamster presents with a moderate, self-limiting disease. Here, we aim to identify relevant mechanisms responsible for the striking differences from a spatial proteomics perspective.

Materials and methods: Hamsters (n = 6) were sacrificed 2 days after intranasal infection with SARS-CoV-2. FFPE lung sections were processed for histology and matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Spatial proteomics data were analyzed with SCiLS™ Lab and Enrichr. Molecular signatures were considered discriminatory with area under curve (AUC) values of <0.3 or >0.7, respectively.

Results: In the Roborovski hamster, 15 proteins were of a higher and six of a lower relative abundance when compared to the Syrian hamster. The former included proteins involved in blood coagulation (annexin A2, fibrinogen alpha chain, myosin-9), cell morphology (plectin isoform X16), and wound healing (heat shock protein beta-1). Strikingly, the latter influenced extracellular matrix morphology (fibronectin isoform X1), leucocyte tethering (vimentin) and respiratory burst (40S ribosomal protein S19). Additionally, some proteins (eg, higher albumin) directly correlated with histomorphology (ie, more severe, protein-rich alveolar oedema).

Conclusions: Spatial proteomics enabled the detection of several discriminating proteins, some of which reflected histomorphological findings. However, the data call for future in-depth proteome studies to allow for a more detailed understanding of distinctive pathways or pathogenetic discrepancies that would explain the stark species differences in disease progression.