Publication Database

Development and analytical validation of a radioimmunoassay for the measurement of alpha1-proteinase inhibitor concentrations in feces from healthy puppies and adult dogs (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Heilmann, Romy M. (WE 20)

Paddock, Casey G.

Ruhnke, Isabelle (WE 15)

Berghoff, Nora

Suchodolksi, Jan S.

Steiner, Jörg M. (WE 20)

Quelle

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians

Bandzählung: 23

Heftzählung: 3

Seiten: 476 – 485

ISSN: 1040-6387

Sprache

Englisch

Verweise

URL (Volltext): https://journals.sagepub.com/doi/10.1177/1040638711404152

DOI: 10.1177/1040638711404152

Pubmed: 21908275

Kontakt

Nutztierklinik: Abteilung Geflügel

Königsweg 63
14163 Berlin
+49 30 838 62676
gefluegelkrankheiten@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Canine α1-proteinase inhibitor (cα1-PI), a proteolysis-resistant protein with a molecular weight similar to albumin, has been shown to be clinically useful as a marker for gastrointestinal protein loss in dogs. A competitive, liquid-phase radioimmunoassay was developed and analytically validated. Fecal samples were collected from 101 healthy pet dogs of various breeds and ages, and fecal cα1-PI (Fcα1-PI) concentrations were compared between dogs of different age groups. A reference interval for Fcα1-PI concentration was calculated using the central 95th percentile. Analytical sensitivity of the assay was 2.2 µg Fcα1-PI/g feces. Observed-to-expected ratios for the serial dilution and spiking recovery of 9 and 6 fecal extracts ranged from 90.4 to 152.0% and from 71.3 to 112.3%, respectively. Coefficients of variation for intra- and interassay variability for 6 fecal extracts were ≤10.8% and ≤12.5%, respectively. The reference intervals for the mean and maximum Fcα1-PI from fecal samples collected on 3 consecutive days were 2.2–13.9 µg/g and 2.2–21.0 µg/g, respectively. Fcα1-PI was significantly higher in dogs <1 year of age (P < 0.0001 for both mean and maximum Fcα1-PI for the 3 samples). The radioimmunoassay described is sensitive, linear, precise, reproducible, and accurate for clinical use, thus allowing reliable quantification of Fcα1-PI in clinical patients. Using this assay, a mean or a maximum Fcα1-PI for 3 sampling days of >13.9 µg/g or >21.0 µg/g, respectively, should be considered abnormal in dogs >1 year of age. Fecal cα1-PI concentrations in dogs <1 year of age were significantly higher and should be carefully interpreted in this age group.