Publikationsdatenbank

Adapter-modified Ussing chamber enables evaluation of endoscopically-obtained colonic biopsy samples from cats and dogs (2012)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ruhnke, Isabelle (WE 15)

DeBiasio, John V.

Suchodolski, Jan S.

Newman, Shelley J.

Musch, Mark W.

Steiner, Jörg M.

Quelle

Research in Veterinary Science

Bandzählung: 93

Heftzählung: 3

Seiten: 1454 – 1461

ISSN: 0034-5288

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S003452881200166X?via%3Dihub

DOI: 10.1016/j.rvsc.2012.05.009

Pubmed: 22721858

Kontakt

Nutztierklinik: Abteilung Geflügel

Königsweg 63
14163 Berlin
+49 30 838 62676
gefluegelkrankheiten@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Adapter-modified Ussing chambers have been used for assessment of endoscopically obtained intestinal biopsies in humans. The aim of this study was to evaluate the feasibility of an adapter-modified Ussing chamber for assessment of intestinal transport physiology in endoscopically-obtained colonic biopsies from cats and dogs. Fifteen colonic biopsies from four cats and 13 colonic biopsies from four dogs were transferred into a modified Ussing chamber and sequentially exposed to several compounds. Baseline mean ± SD conductance was measured. Changes of short circuit current (ΔIsc) were observed after exposure to glucose (number of feline biopsies that responded = 0/number of canine biopsies that responded = 4), phloridzin (n = 0/n = 7), histamine (n = 5/n = 12), serotonin (n = 7/n = 12), prostaglandin (n = 5/n = 7), forskolin (n = 7/n = 7), and ouabain (n = 9/n = 7). The adapter-modified Ussing chamber studied here enables investigation of transport physiology of endoscopically-obtained colonic biopsies from companion animals. However, we observed a large variability of results, suggesting that clinical use of this method is limited.