Publication Database

Evaluation of a method for standardized antimicrobial susceptibility testing with mycoplasma hyorhinis field isolates (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Käbisch, Lisa (WE 7)

Schink, Anne-Kathrin (WE 7)

Höltig, Doris (WE 18)

Verspohl, Jutta

Gyuranecz, Miklós

Spergser, Joachim

Kehrenberg, Corinna

Schwarz, Stefan (WE 7)

Quelle

Microorganisms : open access journal

Bandzählung: 11

Heftzählung: 12

Seiten: 1 – 10

ISSN: 2076-2607

Sprache

Englisch

Verweise

URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/38138024/

DOI: 10.3390/microorganisms11122881

Pubmed: 38138024

Kontakt

Nutztierklinik

Königsweg 65
14163 Berlin
+49 30 838 62261
klauentierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee of Antimicrobial Susceptibility Testing (EUCAST) provide standardized methodologies for antimicrobial susceptibility testing of a wide range of nonfastidious and fastidious bacteria, but so far not for Mycoplasma spp. of animal origin. Recently, a proposed method for the standardized broth microdilution testing of Mycoplasma hyorhinis using commercial Sensititre microtiter plates was presented. In this study, we evaluated this broth microdilution method with 37 field isolates and tested their susceptibility toward the following antimicrobial agents: doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin. The isolates originated from different countries, isolation sites, and years. The broth microdilution method was carried out using a modified Friis broth as the culture and test medium. For macrolides and lincosamides, a bimodal distribution with elevated MIC values could be observed for almost half of the tested field isolates, deducing reduced susceptibility toward these substances. With a recently published protocol, we were able to test a variety of field isolates, and consistent data could be obtained. Using this method, monitoring studies of Mycoplasma hyorhinis isolates can be carried out in a comparable manner, and the observed susceptibility profiles can be screened for possible changes in MIC values in the future.