Publikationsdatenbank

Use of viscous medium to study anthelmintic drug action in Caenorhabditis elegans (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Hellinga, Jacqueline R. (WE 13)

Krücken, Jürgen (WE 13)

Schulenburg, Hinrich

von Samson-Himmelstjerna, Georg (WE 13)

Quelle

Scientific reports

Bandzählung: 14

Heftzählung: 1

Seiten: 12756

ISSN: 2045-2322

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s41598-024-63090-z

DOI: 10.1038/s41598-024-63090-z

Pubmed: 38830930

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Caenorhabditis elegans is an appealing tool for experimental evolution and for working with antiparasitic drugs, from understanding the molecular mechanisms of drug action and resistance to uncover new drug targets. We present a new methodology for studying the impact of antiparasitic drugs in C. elegans. Viscous medium was initially designed for C. elegans maintenance during long-term evolution experiments. Viscous medium provides a less structured environment than the standard nematode growth media agar, yet the bacteria food source remains suspended. Further, the Viscous medium offers the worm population enough support to move freely, mate, and reproduce at a rate comparable to standard agar cultures. Here, the Viscous medium was adapted for use in antiparasitic research. We observed a similar sensitivity of C. elegans to anthelmintic drugs as in standard liquid media and statistical difference to the standard agar media through a larval development assay. Using Viscous medium in C. elegans studies will considerably improve antiparasitic resistance research, and this medium could be used in studies aimed at understanding long-term multigenerational drug activity.