Publikationsdatenbank

Emergence of rapidly spreading antimicrobial-resistant Salmonella in traditional blood-based foods (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Yang, Tian

Wu-Chen, Ricardo A.

Zhang, Runrun

Liao, Xinyu

Lou, Yiyang

Gölz, Greta (WE 8)

Ding, Tian

Feng, Jinsong

Quelle

Food Quality and Safety

Bandzählung: 8

Seiten: 1 – 11

ISSN: 2399-1399

Sprache

Englisch

Verweise

URL (Volltext): https://academic.oup.com/fqs/article/doi/10.1093/fqsafe/fyae009/7607787?login=true

DOI: 10.1093/fqsafe/fyae009

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objectives: This study aims to investigate the survival ability and risk of Salmonella isolated from traditional blood-based food, using both
phtnotypic and genotypic analysis.
Materials and Methods: We characterized and identified seventeen Salmonella isolates using 16s rRNA sequencing, real-time PCR and
whole-genome sequencing (WGS). Cell counts were recorded to monitor growth ability and temperature tolerance. Virulence genes and whole
genome phylogenies were confirmed by WGS. The minimum inhibitory concentration (MIC) of corresponding strains to different antibiotics and
antimicrobial resistance (AMR) genes predicted by WGS were evaluated.
Results: In this study, we characterized 17 Salmonella isolates obtained from contaminated traditional blood-based food in China. Compared
to laboratory strain S. Typhimurium ATCC 14028, these Salmonella isolates generally grew more rapidly and developed less biofilm, but
their tolerance to food processing-associated cold and heat stresses was distinct. Whole-genome phylogenies pointed out the potential for
cross-contamination during food practices. In addition, 21 antibiotic-resistance genes were found among blood-based food isolates, including
high-prevalent resistance genes gyrA (including associated mutations D87G and S83F), blaTEM, and aadA1. Antibiotic susceptibility test con-
firmed the in-silico prediction and revealed a 41.18% (7/17) multidrug resistance (MDR) rate. Resistance to ceftiofur, kanamycin, and nalidixic
acid was observed without corresponding resistance genes, suggesting overlooked resistance mechanisms.
Conclusions: This study revisited microbial safety concerns associated with traditional blood-based food and underlined the emergence of
high-risk MDR Salmonella strains. It also underscores the importance of implementing better hygiene practices in the production and handling
of traditional food products.