Publikationsdatenbank

SARS-CoV-2 tropism to intestinal but not gastric epithelial cells is defined by limited ACE2 expression (2024)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Pauzuolis, Mindaugas

Fatykhova, Diana

Zühlke, Boris

Schwecke, Torsten

Neyazi, Mastura

Samperio-Ventayol, Pilar

Aguilar, Carmen

Schlegel, Nicolas

Dökel, Simon (WE 12)

Ralser, Markus

Hocke, Andreas

Krempl, Christine

Bartfeld, Sina

Quelle

Stem cell reports

Bandzählung: 19

Heftzählung: 5

Seiten: 629 – 638

ISSN: 2213-6711

Sprache

Englisch

Verweise

URL (Volltext): https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(24)00083-3

DOI: 10.1016/j.stemcr.2024.03.008

Pubmed: 38670110

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection primarily affects the lung but can also cause gastrointestinal (GI) symptoms. In vitro experiments confirmed that SARS-CoV-2 robustly infects intestinal epithelium. However, data on infection of adult gastric epithelium are sparse and a side-by-side comparison of the infection in the major segments of the GI tract is lacking. We provide this direct comparison in organoid-derived monolayers and demonstrate that SARS-CoV-2 robustly infects intestinal epithelium, while gastric epithelium is resistant to infection. RNA sequencing and proteome analysis pointed to angiotensin-converting enzyme 2 (ACE2) as a critical factor, and, indeed, ectopic expression of ACE2 increased susceptibility of gastric organoid-derived monolayers to SARS-CoV-2. ACE2 expression pattern in GI biopsies of patients mirrors SARS-CoV-2 infection levels in monolayers. Thus, local ACE2 expression limits SARS-CoV-2 expression in the GI tract to the intestine, suggesting that the intestine, but not the stomach, is likely to be important in viral replication and possibly transmission.