Publication Database

Using population dynamics and transposon-directed insertion site sequencing (TraDIS) to identify bacterial factors essential for the egress from the neonate epithelial cell (2023)

Art

Vortrag

Autoren

Lemke, Christine (WE 7)

van Vorst, Kira (WE 7)

Tedin, Karsten (WE 7)

Aubry, Etienne (WE 7)

Hensel, Michael

Hornef, Mathias

Fulde, Marcus (WE 7)

Kongress

Zoonoses 2023 - International Symposium on Zoonoses Research

Berlin, 09. – 11.10.2023

Quelle

Zoonoses 2023 - International Symposium on Zoonoses Research : benefits and chances of one health research : program and abstracts — German Research Platform for Zoonoses (Hrsg.)

— S. 32

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/260/attachments/99/255/Zoonoses%202023%20-%20Book%20of%20Abstracts.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Non-typhoidal Salmonella (NTS) are a global health problem in human and veterinary medicine. Low hygiene conditions and young age the risk for systemic dissemination. We applied our previously established neonatal mouse model to examine S. Typhimurium pathogenesis and systemic distribution.
Within host cells, NTS typically reside in a specialized membrane-bound compartment, the Salmonella containing vacuole (SCV). To overcome the epithelial barrier, NTS use effector proteins encoded by Salmonella Pathogenicity Islands (SPIs). We recently demonstrated that SPI2 effector proteins play an important role in transmigration of the SCV across intestinal enterocytes. In the present study, our aim is to further identify essential genes for breaching the intestinal barrier, enterocyte egress and subsequent systemic spread. We created a mutant library with random Tn5 transposon integrations and will orally administer it to newborn mice (input pool). We will subsequently compare it to populations isolated from different tissues (output pool). The results of this study will contribute to a better com- prehension of the process of egress and systemic spread and potentially assist in development of new treatment and prevention strategies for NTS infections in the newborn.