Publication Database

The Importance of the RGD motif of Streptococcal protein IdeC in Streptococcus canis infection (2023)

Art

Poster

Autoren

Walsh, Saoirse (WE 7)

Lapschies, Antje M. (WE 7)

Müsken, Mathias

Rohde, Manfred

Bergmann, Simone

Fulde, Marcus (WE 7)

Kongress

Zoonoses 2023 - International Symposium on Zoonoses Research

Berlin, 09. – 11.10.2023

Quelle

Zoonoses 2023 - International Symposium on Zoonoses Research : benefits and chances of one health research : program and abstracts — German Research Platform for Zoonoses (Hrsg.)

— S. 60

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/260/attachments/99/255/Zoonoses%202023%20-%20Book%20of%20Abstracts.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Streptococcus canis is an opportunistic pathogen that predominantly infects cats and dogs. Although,
through contact with companion animals, human infections can also occur. S. canis colonizes the skin
and mucosa of the host and can cause a variety of invasive diseases.
IdeC is an IgG specific protease of S. canis. A secreted protein acts on IgG by cleaving at the hinge
region. IdeC contains an RGD motif; the most common amino acid sequence involved in adhesion to the
extracellular matrix. This motif had been shown in several bacterial proteins to facilitate adhesion or
invasion into host cells. The presence of this motif paired with the ability of IdeC to bind back to the
bacterial surface suggests a possible role for IdeC in adhesion or invasion.
Here, recombinant protein is used to coat fluorescent latex beads, the interactions between these beads
and host cells were then studied.
Based on fluorescence microscopy analysis, there is evidence that IdeC interacts with host cells in a RGD
dependant manor. Further, electron microscopy indicates that IdeC coated beads are internalised by host
cells.
In conclusion, IdeC may have a secondary function in bacterial adhesion and invasion into host cells.