Publikationsdatenbank

Serum and urinary matrix metalloproteinase-9 concentrations in dehydrated horses (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

van Spijk, Julia N.

Lo, Hsiao-Chien (WE 17)

Merle, Roswitha (WE 16)

Richter, Ina-Gabriele

Diemar, Anne

Stoeckle, Sabita D. (WE 17)

Gehlen, Heidrun (WE 17)

Quelle

Animals

Bandzählung: 13

Heftzählung: 24

Seiten: Artikelnummer: 3776

ISSN: 2076-2615

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2615/13/24/3776

DOI: 10.3390/ani13243776

Kontakt

Pferdeklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62299 / 62300
pferdeklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Matrix metalloproteinase-9 is increased in renal tissue in human kidney disease, but its role as a biomarker for kidney disease has not been fully evaluated yet. The aim of this study was to evaluate serum MMP-9 (sMMP-9) and urinary MMP-9 (uMMP-9) concentrations in dehydrated horses. Dehydrated horses were prospectively included. Blood and urinary samples were taken at admission, and after 12, 24, and 48 h (t0, t12, t24, t48), an anti-equine MMP-9 sandwich ELISA was used. Four healthy horses served as the controls. Serum creatinine, urea, symmetric dimethylarginine (SDMA), urine-specific gravity, urinary protein concentration, fractional sodium excretion, and urinary gamma–glutamyl transferase/creatinine ratio (uGGT/Cr) were measured. Statistical analysis included a repeated measures ANOVA and mixed linear regression model. Overall, 40 dehydrated horses were included (mild dehydration 13/40, moderate 16/40, severe 11/40). Acute kidney injury was found in 1/40 horses; 7/40 horses showed elevated serum creatinine, 11/40 horses elevated serum SDMA, and 5/28 elevated uGGT/Cr at presentation. In dehydrated horses, sMMP-9 concentrations were significantly higher on t0 (median: 589 ng/mL, range: 172–3597 ng/mL) compared to t12 (340 ng/mL, 132–1213 ng/mL), t24 (308 ng/mL, 162–1048 ng/mL), and t48 (258 ng/mL, 130–744 ng/mL). In healthy horses, sMMP-9 (239 ng/mL, 142–508 ng/mL) showed no differences over time or compared to patients. uMMP-9 and uMMP-9/creatinine did not differ over time or to the controls. No differences were found between dehydration groups. Urinary casts (p = 0.001; estimate = 135) and uGGT/Cr (p = 0.03; estimate = 6.5) correlated with sMMP-9. Serum urea was associated with uMMP-9/Cr (p = 0.01, estimate 0.9). In conclusion, sMMP-9 was elevated at arrival in dehydrated patients compared to later measurements. Correlations to uGGT/Cr and urinary casts need further evaluation.