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This document represents a synthesis of discussions held online at the Second Koala Retrovirus Workshop in 2021. The three days of discussions were based on workshop presentations and comprise: KoRV foundational science (Day 1); applied management of koalas in zoo populations (Day 2); and applied management of koalas in wild populations (Day 3). Each of these discussions gathers current knowledge, explores points of consensus and disagreement, and identifies important knowledge gaps. Recommendations arise regarding research strategy, interim measures for management, and support of research and management via initiation of working groups on KoRV diagnostics and biobanking.