Publikationsdatenbank

Detection of “VBNC-Campylobacter” in broiler farm environments:
insights from PMA-qPCR analysis (2023)

Art

Poster

Autoren

Reichelt, Benjamin (WE 10)

Stingl, Kerstin

Roesler, Uwe (WE 10)

Friese, Anika (WE 10)

Kongress

Zoonoses 2023 - International Symposium on Zoonoses Research

Berlin, 09. – 11.10.2023

Quelle

Zoonoses 2023 - International Symposium on Zoonoses Research : benefits and chances of one health research : program and abstracts — German Research Platform for Zoonoses (Hrsg.)

— S. 115

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/260/attachments/99/255/Zoonoses%202023%20-%20Book%20of%20Abstracts.pdf

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Studies suggest that Campylobacter can enter a viable but non-culturable (VBNC) state. The significance of this state form for a spread on farms is unclear. To investigate this, a PMA dye-supported viability qPCR approach was used to examine environmental matrices on culturable Campylobacter and “VBNC-Campylobacter” in broiler farm environment and in an experimental animal pen.
Seven visits to broiler farms were conducted, collecting environmental and barn samples. Sampling was also done in an animal facility after removing Campylobacter-positive animals. Chicken manure from five trials was examined for 72 hours. Campylobacter was cultured and if this failed, putative VBNC cells were analyzed using live-dead discrimination.
At three sampling times no Campylobacter at all was found inside or outside the chicken barn in all 72 samples. At the other four sampling times, it was present in the barn and also in environmental samples from outside (15.9% positive for “VBNC-Campylobacter”, 62.2% Campylobacter DNA-positive, 1.2% culturable C.jejuni).
In the experimental animal facility manure from five chicken groups was negative for culturable C.jejuni after 24 h. In contrast, after 72 h “VBNC-Campylobacter” were found in many manure samples.
“VBNC-Campylobacter” was found in broiler barns´environment and inside a barn during experiments. However, further research is needed to determine long-term sustainability in broiler farms.