Publikationsdatenbank

Characterization of an optrA-harbouring unconventional circularizable structure located on the novel integrative and conjugative element ICESsuHN38 in Streptococcus suis (2023)

Art

Poster

Autoren

Yang, Qin

Zhu, Yao

Schwarz, Stefan (WE 7)

Zhang, Wanjiang

Wang, Xiumei

Kongress

ARAE 2023

Tours, Frankreich, 03. – 05.07.2023

Quelle

9th Symposium on Antimicrobial Resistance in Animals and the Environment : abstracts book

Tours, Frankreich, 2023 — S. 130

Sprache

Englisch

Verweise

URL (Volltext): https://arae2023.symposium.inrae.fr/content/download/770/8117?version=1

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objectives: To identify the novel genetic elements involved in the horizontal transfer of the oxazolidinone/phenicol resistance gene optrA in Streptococcus suis.
Methods: Whole genome DNA of the optrA-positive isolate S. suis HN38 was subjected to WGS via both Illumina HiSeq and Oxford Nanopore platforms. MICs of several antimicrobial agents were determined by broth microdilution. PCR assays were performed to identify the circular forms of the novel integrative and conjugative element (ICE) ICESsuHN38, but also the unconventional circularizable structure (UCS) excised from this ICE. The transferability of ICESsuHN38 was evaluated by conjugation assays.
Results: S. suis isolate HN38 harbored the oxazolidinone/phenicol resistance gene optrA. The optrA gene was flanked by two copies of erm(B) genes in the same orientation, located on a novel ICESa2603 family-like ICE, designated ICESsuHN38. PCR assays revealed that a novel UCS carrying the optrA and one copy of erm(B) could be excised from ICESsuHN38. ICESsuHN38 was able to successfully transfer into the recipient strain S. suis BAA by conjugation.
Conclusions: In this work, a novel optrA-carrying mobile genetic element, an UCS, was identified in S. suis. The optrA gene was flanked by copies of erm(B) and its location on the novel ICESsuHN38 will aid its horizontal dissemination.