Publication Database

Linezolid-resistant Enterococcus faecalis of chicken origin harbored chromosome-borne optrA and plasmid-borne cfr, cfr(D) and poxtA2 genes (2023)

Art

Poster

Autoren

Du, Xiang-Dang

Yao, Hong

Xu, Chunyan

Li, Chenglong

Biao, Tang

Zhao, Jinfeng

Shan, Xinxin

Schwarz, Stefan (WE 7)

Kongress

ARAE 2023

Tours, Frankreich, 03. – 05.07.2023

Quelle

9th Symposium on Antimicrobial Resistance in Animals and the Environment : abstracts book

Tours, Frankreich, 2023 — S. 105

Sprache

Englisch

Verweise

URL (Volltext): https://arae2023.symposium.inrae.fr/content/download/770/8117?version=1

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The aim of this study was to investigate the transferability of acquired linezolid resistance genes and associated mobile genetic elements in an Enterococcus faecalis isolate QZ076, co-carrying optrA, cfr, cfr(D) and poxtA2 genes. MICs were determined by broth microdilution. WGS was performed using the Illumina and Nanopore platforms. The transfer of linezolid resistance genes was investigated by conjugation, using E. faecalis JH2-2 and clinical MRSA 109 as recipients. E. faecalis QZ076 harbors four plasmids, designated pQZ076-1 to pQZ076-4, with optrA located in the chromosomal DNA. The gene cfr was located on a novel pseudo-compound transposon, designated Tn7515, integrated into the 65,961-bp pCF10-like pheromone-responsive conjugative plasmid pQZ076-1. Tn7515 generated 8-bp direct target duplications (5’-GATACGTA-3’). The genes cfr(D) and poxtA2 were co-located on the 16,397-bp mobilizable broad-host-range Inc18 plasmid pQZ076-4. The cfr-carrying plasmid pQZ076-1 could transfer from E. faecalis QZ076 to E. faecalis JH2-2, along with the cfr(D)- and poxtA2-co-carrying plasmid pQZ076-4, conferring the corresponding resistant phenotype to the recipient. Moreover, pQZ076-4 could also transfer to MRSA 109. To the best of our knowledge, this study presented the first report of four acquired linezolid resistance genes [optrA, cfr, cfr(D), poxtA2] being simultaneously present in the same E. faecalis isolate. The location of the cfr gene on a pseudo-compound transposon in a pheromone-responsive conjugative plasmid will accelerate its rapid dissemination. In addition, the cfr-carrying pheromone-responsive conjugative plasmid in E. faecalis was also able to mobilize the inter-species transfer of the cfr(D)- and poxtA2-co-carrying plasmid between enterococci and staphylococci.