Publikationsdatenbank

Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM):
the next frontier of drug discovery and disease understanding (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Barroso, Margarida

Monaghan, Michael G.

Niesner, Raluca A. (WE 2)

Dmitriev, Ruslan I.

Quelle

Advanced drug delivery reviews

Bandzählung: 201

Seiten: 115081

ISSN: 1872-8294

Sprache

Englisch

Verweise

URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/37647987/

DOI: 10.1016/j.addr.2023.115081

Pubmed: 37647987

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Organoid models have been used to address important questions in developmental and cancer biology, tissue repair, advanced modelling of disease and therapies, among other bioengineering applications. Such 3D microenvironmental models can investigate the regulation of cell metabolism, and provide key insights into the mechanisms at the basis of cell growth, differentiation, communication, interactions with the environment and cell death. Their accessibility and complexity, based on 3D spatial and temporal heterogeneity, make organoids suitable for the application of novel, dynamic imaging microscopy methods, such as fluorescence lifetime imaging microscopy (FLIM) and related decay time-assessing readouts. Several biomarkers and assays have been proposed to study cell metabolism by FLIM in various organoid models. Herein, we present an expert-opinion discussion on the principles of FLIM and PLIM, instrumentation and data collection and analysis protocols, and general and emerging biosensor-based approaches, to highlight the pioneering work being performed in this field.