Publikationsdatenbank

Insight into metabolic properties of porcine LA-MRSA CC9 and CC398 isolates from China and Germany via Biolog Phenotype MicroArray™ (2023)

Art

Poster

Autoren

Krüger-Haker, H. (WE 7)

Ji, X.

Bartel, A.

Feßler, A. T. (WE 7)

Hanke, D. (WE 7)

Jiang, N.

Tedin, K. (WE 7)

Maurischat, S.

Wang, Y.

Wu, C.

Schwarz, S. (WE 7)

Kongress

Tagung der DVG-Fachgruppe Bakteriologie & Mykologie

Berlin, 22. – 24.05.2023

Quelle

Tagung der DVG-Fachgruppe Bakteriologie & Mykologie : 22. bis 24. Mai 2023 — Wissenschaftliche Leitung: Prof. Dr. Stefan Schwarz & Prof. Dr. Marcus Fulde, Berlin Veranstalter und Organisation: DVG Service GmbH, Gießen (Hrsg.)

1. Auflage

Gießen: Verlag der DVG Service GmbH, 2023 — S. 97–99

ISBN: 978-3-86345-666-5

Sprache

Englisch

Verweise

URL (Volltext): https://www.dvg.net/fileadmin/Bilder/DVG/PDF/Gesamtdatei_Tagungsband_Bak-Myk_2023.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is an important zoonotic pathogen and often multiresistant to antimicrobial agents. In swine, the dominant LA-MRSA in Asia belong to clonal complex (CC) 9 and in Europe, Australia and the Americas to CC398. We compared the metabolic properties of rare and widespread porcine LA-MRSA from Germany and China to evaluate if metabolic differences could have played a role in the evolution of the two different epidemic LA-MRSA lineages.

Methodology:
In total, 20 porcine LA-MRSA-CC9 and -CC398 from China and Germany were investigated using Biolog Phenotype MicroArray™ technology in at least two runs. Metabolic responses to carbon sources, osmolytes andvarying pH conditions were quantified considering area under the curve, lag time, maximum height, plateau time and slope of the respiration curves. Sparse partial least squares discriminant analysis (sPLS-DA) was applied to determine the most informative out of 379 substances and/or conditions tested and enabled isolate differentiation on the basis of CC and origin. A heat map was generated based on the selected variables and parameters were ranked according to variable importance.

Results:
Overall, the LA-MRSA were tolerant to varying environmental conditions. The sPLS-DA revealed metabolic differences between the LA-MRSA groups.
Only few metabolic variations were found between the dominant and rare lineages within the same CC. However, the Chinese LA-MRSA-CC9 displayed
major metabolic differences compared to the other isolates. In addition to the increased, reduced or absent usage of several nutrients, especially the de-
layed onset of cellular respiration was often noticed. The heat map showing clustering of the isolates into four groups based on CC and origin also sug-
gested a unique metabolic profile for the LA-MRSA-CC9 from China.

Conclusions:
Unique metabolic properties most likely did not play a crucial role in the development of the respective dominant CC9 and CC398 lineages in China and Germany, respectively. However, the possibly unfavorable metabolic properties of the Chinese LA-MRSA-CC9 might promote the suspected already ongoing gradual replacement of this current epidemic lineage in China by the emerging MRSA-CC398 through trading activities and occupational
exposure. Thus, the public health risk posed by porcine LA-MRSA might increase further due to the enhanced pathogenicity of MRSA-CC398.