Publikationsdatenbank

Diversity of methicillin-susceptible Staphylococcus aureus (MSSA) isolates from various companion and wild animals (2023)

Art

Vortrag

Autoren

Loncaric, Igor

Cabal, Rosel

Szostak, M.P.

Künzel, F.

Licka, T.

Mierer, J.

Kübber-Heiss, A.

Müller, E.

Braun, S.D.

Ehling-Schulz, M.

Feßler, Andrea T (WE 7)

Schwarz, Stefan (WE 7)

Gunert, T.

Ehricht, R.

Monecke, S.

Ruppitsch, W.

Spergser, J.

Kongress

Tagung der DVG-Fachgruppe Bakteriologie und Mykologie

Berlin, 22. – 24.05.2023

Quelle

Tagung der DVG-Fachgruppe Bakteriologie und Mykologie : 22. bis 24. Mai 2023 / — Deutsche Veterinärmedizinische Gesellschaft e.V. ; wissenschaftliche Leitung: Prof. Dr. Stefan Schwarz (Berlin), Prof. Dr. Marcus Fulde (Berlin). (Hrsg.)

1. Auflage

Gießen: Verlag der DVG Service GmbH, 2023 — S. 3–4

ISBN: 978-3-86345-666-5

Sprache

Englisch

Verweise

URL (Volltext): https://d-nb.info/1289336938/04

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Staphylococcus (S.) aureus is an important pathogen that infect multiple hosts ranging from humans to domestic and wild animals. It is capable of causing a wide spectrum of clinical manifestations in humans and animals.
Extensive knowledge exists on methicillin resistant (MRSA) and methicillin susceptible (MSSA) S. aureus, associated with infections in humans.
On the contrary, much less information is available, especially on MSSA, associated with clinical manifestations in companion animals and wildlife species.

Materials and Methods:
During the present study, 82 non-repetitive clinical MSSA isolates originating from horses (n=25), dogs (n=19), cats (n=18), rabbits (n=3), ducks (n=3), roe deers (n=2), rats (n=2), a turtle, a guinea pig, a grey parrot, a red deer, a sparrow, a donkey, a chicken, a chinchilla, a chamois, and a bearded dragon were obtained during diagnostic activities. MSSA was identified by matrix-assisted laser desorption-ionization-time of flight mass spectrometry. Susceptibility testing was performed by agar disk diffusion.
MSSA isolates were further characterized by DNA microarray-based anal-ysis, spa typing, and whole-genome sequencing of selected isolates.

Results:
Among resistant isolates, penicillin-resistance (blaZ) alone was most frequently detected. Two MSSA isolates exhibited a multi-resistance phenotype (penicillin/gentamicin/tetracycline) that correlated with the resistance genotype (blaZ /aacA-aphD/tet(K)). Forty-six different known and several novel spa types were detected, with t008 being predominant (n=5). Among isolates that were whole-genome sequenced, numerous novel sequence types (ST) were detected as well as 30 known STs with ST8 (n=7) being the most common. DNA microarray differentiated 22 clonal complexes (CC). In addi-tion, four isolates could not be assigned to any known CC. The presence of several virulence genes was observed. The toxic shock syndrome toxin 1 gene (tst) was detected in eight isolates, whereas 27 isolates harbored the enterotoxin gene cluster (egc) comprising various enterotoxin genes.

Conclusion:
This study presents the first detailed analysis of MSSA isolates from Austrian companion animals. Considering the high proximity between companion animals and humans and the presence of some human-associated clones in both companion animals and wildlife, our results identified a public health issue that emphasizes the need for a One Health approach and constant surveillance.