Publikationsdatenbank

Genomic diversity of porcine LA-MRSA CC398 isolates collected in the German national resistance monitoring program GERM-Vet between 2007 and 2019 (2023)

Art

Vortrag

Autoren

Krüger-Haker, Henrike (WE 7)

Ji, Xiang

Hanke, Dennis (WE 7)

Fiedler, Stefan

Feßler, Andrea T. (WE 7)

Jiang, Nansong

Kaspar, Heike

Wang, Yang

Wu, Congming

Schwarz, Stefan (WE 7)

Kongress

ARAE 2023

Tours, Frankreich, 03. – 05.07.2023

Quelle

9th Symposium on Antimicrobial Resistance in Animals and the Environment : abstracts book

Tours, Frankreich, 2023 — S. 27

Sprache

Englisch

Verweise

URL (Volltext): https://arae2023.symposium.inrae.fr/content/download/770/8117?version=1

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives: The livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 lineage is known for its frequent multiresistance to antimicrobial agents and a low host specificity. Colonized pigs represent a risk of LA-MRSA CC398 colonization or infection for occupationally exposed people, through which such isolates might be spread further within the human community. Here, we investigated a collection of unrelated LA-MRSA CC398 isolates for evolutionary relationships and genomic diversity with focus on their virulence and antimicrobial resistance (AMR) properties.
Materials and methods: In total, 178 unrelated LA-MRSA CC398 were collected in the German national resistance monitoring program GERM-Vet from diseased swine between 2007 and 2019. Whole-genome sequencing was carried out, followed by molecular typing and sequence analysis. A minimum spanning tree based on core-genome multilocus sequence typing was constructed and antimicrobial susceptibility testing was performed according to CLSI standards.
Results: The LA-MRSA CC398 isolates showed close phylogenetic relationships and a wide molecular variety, including 13 spa types as well as 19 known and four novel dru types. Several toxin-encoding genes, including eta, seb, sek, sep and seq, were detected. The isolates harbored a wide range of AMR properties mirroring the proportions of the classes of antimicrobial agents applied in veterinary medicine in Germany. Several novel or rare AMR genes were identified, including the novel macrolide-lincosamide-streptogramin B resistance gene erm(54), the lincosamide-pleuromutilin-streptogramin A resistance gene vga(C), and the phenicol-lincosamide-oxazolidinone-pleuromutilin-streptogramin A resistance gene cfr. Many AMR genes were part of small transposons or plasmids. Clonal and geographical correlations of molecular characteristics, AMR and virulence genes were more frequently observed than temporal relations.
Conclusion: This study provided insight into diversity and population dynamics of the epidemic porcine LA-MRSA lineage in Germany over a 13-year-period. In order to detect new emerging, possibly more dangerous clones and to prevent LA-MRSA transmission between livestock farms and entries into the human community, a large-scale LA-MRSA monitoring is essential.