Publication Database

Effects of TNF and Cannabidiol on porcine intestinal IPEC-J2 cells (2023)

Art

Poster

Autoren

Boehm, Elisa (WE 2)

Drößler, Linda (WE 2)

Trappe, Susanne (WE 2)

Amasheh, Salah (WE 2)

Kongress

77. Tagung der Gesellschaft für Ernährungsphysiologie

Göttingen, 07. – 09.03.2023

Quelle

Proceedings of the Society of Nutrition Physiology — GfE (Hrsg.)

Frankfurt: DLG Verlag, 2023; 32 — S. 85

ISBN: 978-3-7690-4116-3

Sprache

Englisch

Verweise

URL (Volltext): https://www.openagrar.de/receive/openagrar_mods_00088797

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In recent years cannabidiol (CBD) gained major attention in manyfold studies, because of its anti-inflammatory, anti-convulsing and analgetic effects without being psychotropic. CBD, extracted from Cannabis sativa, can exert its effect by binding to cannabinoid receptors [1]. Beneficial effects of CBD have already been demonstrated in different models, but it remains unknown whether these effects also occur in porcine enterocytes. Recently it has been shown that proinflammatory cytokine Tumor Necrosis Factor alpha (TNFα)-treated porcine jejunal epithelial IPEC-J2 cells serve as an appropriate inflammatory model of the intestinal epithelium [2,3], showing a disturbed barrier function. Therefore, this study was performed to investigate whether CBD can prevent the barrier weakening effect of TNFα, rusing IPEC-J2 cells.

Methods:
IPEC-J2 cells were grown on semipermeable cell culture inserts until confluency.For analyzing the CBD effects under inflammatory conditions, 1000 U/ml TNFα was added to the basolateral compartment, and different concentrations of CBD were added apically. To investigate the epithelial barrier function, the transepithelial resistance was measured for 48 h, employing an epithelial volt-ohm meter. Statistical analysis was performed using JMP 16 software. Data were compared by using Kruskal-Wallis test and statistical significance was determined by Dunnett’s post hoc test. Statistical significance was assumed at values below p = 0.05.

Results:
Sole incubation with1000 U/ml TNFα led to a significant decrease in transepithelial resistance over 48 h compared to controls (TNF: 69.64 ± 2.76 %; ctrl: 89.89 ± 2.01 %; ***p < 0.001, n = 16). 40 μM CBD mitigated this effect induced by TNFα (TNF + 40 μM CBD: 84.72 ± 4.58 %; **p < 0.01, n= 16). Thus, there was no significant difference observed between controls and TNF / 40 μM CBD treated cells after 48 h.

Conclusions:
Our study reveals that CBD can prevent a decrease of transepithelial resistance induced by TNF, indicating a beneficial effect under inflammatory conditions. Further investigations to identify possible effects on intestinal epithelial barrier proteins as an underlying molecular mechanism are in progress.