Publikationsdatenbank

Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ivanusic, Daniel

Denner, Joachim (WE 5)

Quelle

MicroPublication : Biology

Bandzählung: 2023

Seiten: 1

ISSN: 2578-9430

Sprache

Englisch

Verweise

URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/37485021/

DOI: 10.17912/micropub.biology.000773

Pubmed: 37485021

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Lipopolysaccharide (LPS) contaminations may falsify immunological experiments and are crucial for pharmaceutical products because they cause life-threatening immune reactions. Here, we present interleukin-10 (IL-10) as a reliable marker to measure LPS contents when the readout of pro-inflammatory cytokines is not favored. This animal free source assay is able to detect LPS with a limit of detection (LOD) of 0.024 EU/ml by monitoring IL-10 secretions from isolated human peripheral blood mononuclear cells (PBMCs).