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    The large extracellular loop is important for recruiting CD63 to exosomes (2023)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Ivanusic, Daniel
    Denner, Joachim (WE 5)
    Quelle
    MicroPublication : Biology
    Bandzählung: 2023
    Seiten: e12788
    ISSN: 2578-9430
    Sprache
    Englisch
    Verweise
    URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/37602284/
    DOI: 10.17912/micropub.biology.000842
    Pubmed: 37602284
    Kontakt
    Institut für Virologie

    Robert-von-Ostertag-Str. 7-13
    14163 Berlin
    +49 30 838 51833
    virologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Exosomes are small extracellular vesicles that are secreted from cells. To characterize exosome fraction marker proteins of the tetraspanin family in particular, CD9, CD63, and CD81 are routinely used. CD63 expression constructs were employed to investigate the influence of the large extracellular loop (LEL) of CD63 on sorting into exosomes. When the LEL of CD63 fused with mCherry was deleted, the protein was no longer found in the purified exosome fraction. This finding demonstrates the importance of the LEL sequence for the recruitment of CD63 into exosomes.