Publikationsdatenbank

Correction: State-of-the-art analytical methods of viral infections in human lung organoids (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Baumgardt, Morris

Hülsemann, Maren

Löwa, Anna

Fatykhova, Diana

Hoffmann, Karen

Kessler, Mirjana

Mieth, Maren

Hellwig, Katharina

Frey, Doris

Langenhagen, Alina (WE 12)

Voss, Anne

Obermayer, Benedikt

Wyler, Emanuel

Dökel, Simon (WE 12)

Gruber, Achim D. (WE 12)

Tölch, Ulf

Hippenstiel, Stefan

Hocke, Andreas C.

Hönzke, Katja

Quelle

PLOS ONE

Bandzählung: 18

Heftzählung: 11

Seiten: e0294216

ISSN: 1932-6203

Sprache

Englisch

Verweise

URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/37922305/

DOI: 10.1371/journal.pone.0294216

Pubmed: 37922305

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

[This corrects the article DOI: 10.1371/journal.pone.0276115.].

Human-based organ models can provide strong predictive value to investigate the tropism, virulence, and replication kinetics of viral pathogens. Currently, such models have received widespread attention in the study of SARS-CoV-2 causing the COVID-19 pandemic. Applicable to a large set of organoid models and viruses, we provide a step-by-step work instruction for the infection of human alveolar-like organoids with SARS-CoV-2 in this protocol collection. We also prepared a detailed description on state-of-the-art methodologies to assess the infection impact and the analysis of relevant host factors in organoids. This protocol collection consists of five different sets of protocols. Set 1 describes the protein extraction from human alveolar-like organoids and the determination of protein expression of angiotensin-converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2) and FURIN as exemplary host factors of SARS-CoV-2. Set 2 provides detailed guidance on the extraction of RNA from human alveolar-like organoids and the subsequent qPCR to quantify the expression level of ACE2, TMPRSS2, and FURIN as host factors of SARS-CoV-2 on the mRNA level. Protocol set 3 contains an in-depth explanation on how to infect human alveolar-like organoids with SARS-CoV-2 and how to quantify the viral replication by plaque assay and viral E gene-based RT-qPCR. Set 4 provides a step-by-step protocol for the isolation of single cells from infected human alveolar-like organoids for further processing in single-cell RNA sequencing or flow cytometry. Set 5 presents a detailed protocol on how to perform the fixation of human alveolar-like organoids and guides through all steps of immunohistochemistry and in situ hybridization to visualize SARS-CoV-2 and its host factors. The infection and all subsequent analytical methods have been successfully validated by biological replications with human alveolar-like organoids based on material from different donors.