Publication Database

Evaluation of tissue factor expression in canine tumor cells (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Stokol, Tracy

Daddona, Janelle

Mubayed, Lamya

Trimpert, Jakob (WE 5)

Kang, Sungkwon

Quelle

American journal of veterinary research : AJVR

Bandzählung: 72

Heftzählung: 8

Seiten: 1097 – 1106

ISSN: 0002-9645

Sprache

Englisch

Verweise

URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/21801068/

DOI: 10.2460/ajvr.72.8.1097

Pubmed: 21801068

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objective:
To determine whether canine tumor cell lines express functional tissue factor and shed tissue factor-containing microparticles.

Sample:
Cell lines derived from tumors of the canine mammary gland (CMT12 and CMT25), pancreas (P404), lung (BACA), prostate gland (Ace-1), bone (HMPOS, D-17, and OS2.4), and soft tissue (A72); from normal canine renal epithelium (MDCK); and from a malignant human mammary tumor (MDA-MB-231).

Procedures:
Tissue factor mRNA and antigen expression were evaluated in cells by use of canine-specific primers in a reverse transcriptase PCR assay and a rabbit polyclonal anti-human tissue factor antibody in flow cytometric and immunofluorescent microscopic assays, respectively. Tissue factor procoagulant activity on cell surfaces, in whole cell lysates, and in microparticle pellets was measured by use of an activated factor X-dependent chromogenic assay.

Results:
Canine tissue factor mRNA was identified in all canine tumor cells. All canine tumor cells expressed intracellular tissue factor; however, the HMPOS and D-17 osteosarcoma cells lacked surface tissue factor expression and activity. The highest tissue factor expression and activity were observed in canine mammary tumor cells and pulmonary carcinoma cells (BACA). These 3 tumors also shed tissue factor-bearing microparticles into tissue culture supernatants.

Conclusions and clinical relevance:
Tissue factor was constitutively highly expressed in canine tumor cell lines, particularly those derived from epithelial tumors. Because tumor-associated tissue factor can promote tumor growth and metastasis in human patients, high tissue factor expression could affect the in vivo biological behavior of these tumors in dogs.